CAJ | 학술논문

　　将黑斑蛙蝌蚪暴露于不同体积分数100％、20％、4％、0．8％和0％的工业污水中饲养，观察其生长发育过程，每7 d 记录平均体质量和平均体长及黑斑蛙的成活情况。对照组0％至100％工业污水处理后，蝌蚪的死亡率7 d 内，由3％升至100％；14 d 内，蝌蚪体长平均增长率由43．7％降至21．1％，每增高5倍体积分数，体长平均增长率降低5．6％；蝌蚪体质量平均增长率的影响由238．5％降至135．6％，每增高5倍体积分数，体质量平均增长率降低25．7％。采用单细胞凝胶电泳技术，利用CASP 软件检测分析工业污水对黑斑蛙蝌蚪血细胞 DNA 损伤。结果表明，DNA 损伤呈现剂量效应和时间效应，彗星图像拖尾增加，尾长、尾部 DNA 含量和 Olive 尾距显著增加。因此，本研究可为水质监测、水产养殖等领域提供科学依据和理论基础。
　　장흑반와과두폭로우불동체적분수100％、20％、4％、0．8％화0％적공업오수중사양，관찰기생장발육과정，매7 d 기록평균체질량화평균체장급흑반와적성활정황。대조조0％지100％공업오수처리후，과두적사망솔7 d 내，유3％승지100％；14 d 내，과두체장평균증장솔유43．7％강지21．1％，매증고5배체적분수，체장평균증장솔강저5．6％；과두체질량평균증장솔적영향유238．5％강지135．6％，매증고5배체적분수，체질량평균증장솔강저25．7％。채용단세포응효전영기술，이용CASP 연건검측분석공업오수대흑반와과두혈세포 DNA 손상。결과표명，DNA 손상정현제량효응화시간효응，혜성도상타미증가，미장、미부 DNA 함량화 Olive 미거현저증가。인차，본연구가위수질감측、수산양식등영역제공과학의거화이론기출。
Tadpoles of frog Rana nigromaculata were reared in the water containing (in volume) 100% , 20% ,4% ,0 .8% and 0% of industrial effluent and average body weight ,body length and survival of the tadpoles were measured once every 7 days .The results showed that mortality rate was decreased from 3%in the control group to 100% in the 100% effluent group .Corresponding , the body length gain was descended from 43 .7% to 21 .1% ,at an average descended rate of 5 .6% with increase in the effluent volume at a rate of 5% during 14 days .The body weight gain was decreased from 238 .5% to 135 .6% ,at an average descended rate of 25 .7% with increase in the effluent volume at a rate of 5% during 14 days . The single cell gel electrophoresis (SCGE) was used to detect the breakage of DNA strand in hemocytes and the software of CASP was used to analyze the date picture .It was found that the DNA damage was shown to be of dose-effect and time-effect dependent .There was a significant increase in the levels of the damage of DNA ,as well as the tail length ,tail DNA ,and olive tail moment (OTM ) .The findings indicate that R .nigromaculata tadpoles can be used to monitor water pollution ,and that the present study is a foundation for the protection of amphibians and other aquatic organisms .