CAJ | 학술논문

　　15℃下测定了不同盐度（15、20、25、30、35）对体质量（87．22±20．71） g 的日本蟳免疫相关指标（血细胞密度、血蓝蛋白含量、酚氧化酶、溶菌酶、超氧化物歧化酶和过氧化氢酶活力、过氧化代谢产物丙二醛含量）和中肠腺消化酶（酸性蛋白酶、碱性蛋白酶、淀粉酶、纤维素酶和脂肪酶）活力的影响。试验结果表明，血细胞密度与盐度呈正相关，盐度对血蓝蛋白含量无显著影响。盐度30组的酚氧化酶活力、盐度20和30组的溶菌酶活力先升后降，而盐度15、20和35组的酚氧化酶活力、盐度15和35组的溶菌酶活力先降后升再降。至处理第5 d ，各试验组的酚氧化酶与溶菌酶的活力为：盐度25＞20＞30＞15＞35。盐度变化后，超氧化物歧化酶和过氧化氢酶活力均表现为先升后降，而丙二醛含量先降后升，至处理第5 d ，以盐度15和35组的超氧化物歧化酶和过氧化氢酶活力较低，而丙二醛含量显著高于其他试验组（P＜0．05）。盐度20组的淀粉酶与纤维素酶活力最高，盐度25组的酸性蛋白酶和脂肪酶活力最高，盐度30组的碱性蛋白酶活力最高，而盐度15组的5种消化酶活力均为最低。盐度20～30为日本蟳较适宜的环境条件，而盐度15与35条件下会引起其免疫能力和消化酶活力的明显降低。超氧化物歧化酶活力和丙二醛含量长期变化情况可作为衡量日本蟳在盐度变化下免疫状态的指标。
　　15℃하측정료불동염도（15、20、25、30、35）대체질량（87．22±20．71） g 적일본심면역상관지표（혈세포밀도、혈람단백함량、분양화매、용균매、초양화물기화매화과양화경매활력、과양화대사산물병이철함량）화중장선소화매（산성단백매、감성단백매、정분매、섬유소매화지방매）활력적영향。시험결과표명，혈세포밀도여염도정정상관，염도대혈람단백함량무현저영향。염도30조적분양화매활력、염도20화30조적용균매활력선승후강，이염도15、20화35조적분양화매활력、염도15화35조적용균매활력선강후승재강。지처리제5 d ，각시험조적분양화매여용균매적활력위：염도25＞20＞30＞15＞35。염도변화후，초양화물기화매화과양화경매활력균표현위선승후강，이병이철함량선강후승，지처리제5 d ，이염도15화35조적초양화물기화매화과양화경매활력교저，이병이철함량현저고우기타시험조（P＜0．05）。염도20조적정분매여섬유소매활력최고，염도25조적산성단백매화지방매활력최고，염도30조적감성단백매활력최고，이염도15조적5충소화매활력균위최저。염도20～30위일본심교괄의적배경조건，이염도15여35조건하회인기기면역능력화소화매활력적명현강저。초양화물기화매활력화병이철함량장기변화정황가작위형량일본심재염도변화하면역상태적지표。
The changes in some immunity-related indicators and digestive enzyme activities were tested in hemolymph and hepatopancreas of Asian swimming crab Charybdis j aponica collected on 0 ,0 .5 ,1 ,2 ,3 , 4 and 5 days under various salinities of 15 ,20 ,25 ,30 and 35 to evaluate the effects of salinity on density of hemocyte (DHC ) , hemocyanin content , activities of the phenoloxidase (PO ) , lysozyme (LSZ ) , superoxide dismutase (SOD) and catalase (CAT ) ,and malondialdehyde (MDA ) content and activities of acid protease ,alkali protease ,lipase ,amylase and cellulase .The results showed that DHC was found to be correlated positively with salinity ,no significant effect on hemocyanin content .The PO activity at a salinity of 30 and LSZ activity at a salinity of 20 and 30 were shown to be increase first and then decline , while the PO activity in a salinity of 15 ,20 and 35 ,and the LSZ activity in a salinity of 15 and 35 were first decreased , then increased , and dropped again . After 5 days , the activities of PO and LSZ were descendantly arranged as salinity 25 > 20 > 30 > 15 > 35 . The activities of SOD and CAT were first increased and then decreased ,the opposite tendency being the change in MDA content .On day 5 ,the activities of SOD and CAT were lower in a salinity of 15 and 35 than those in other treatments with higher MDA content (P < 0 .05) .The maximal activities of amylase and cellulase were observed in a salinity of 20 ,the maximal activities of acid protease and lipase in a salinity of 25 ,and the maximal alkali protease in a salinity of 30 .The minimal activities of 5 digestive enzymes were found in a salinity of 15 .The findings indicated that Asian swimming crab had a suitable salinity of 20 ~ 30 for immunity ,with significantly lower activities of digestive enzymes under a salinity of 15 and 35 .It is suggested that variation in activity of SOD and content of MDA be used as an indicator for immune state of Asian swimming crab under different salinity .