CAJ | 학술논문

　　为了建立黄淮白山羊瘤胃甲烷菌快速检测的PCR方法，采集安徽本地黄淮白山羊瘤胃内容物，进行瘤胃甲烷菌的分离培养，利用瘤胃甲烷杆菌的保守基因甲基辅酶M还原酶基因（MCR）进行引物设计，并且优化PCR扩增条件。结果表明：所设计的引物具有特异性、专一性，优化的PCR扩增条件符合瘤胃甲烷杆菌扩增要求，扩增产物在140 bp的位置出现单一明亮条带。由此可见，利用PCR技术，可以快速、准确检测黄淮白山羊瘤胃甲烷杆菌。
　　위료건립황회백산양류위갑완균쾌속검측적PCR방법，채집안휘본지황회백산양류위내용물，진행류위갑완균적분리배양，이용류위갑완간균적보수기인갑기보매M환원매기인（MCR）진행인물설계，병차우화PCR확증조건。결과표명：소설계적인물구유특이성、전일성，우화적PCR확증조건부합류위갑완간균확증요구，확증산물재140 bp적위치출현단일명량조대。유차가견，이용PCR기술，가이쾌속、준학검측황회백산양류위갑완간균。
An experiment was conduced to establish PCR technique to monitor methanogens of Huanghuai white goat.The rumen contents were collected from local Huanghuai white goat in Anhui to culture Methanobrevibacter ruminantium, and MCR gene sequences were amplified and optimized by PCR amplification.The primer is specific and the condition of amplifica-tion of PCR is suitable for reaction.A light bandofby the product appears at 140 bp.Therefore, PCR on M.ruminantium in rumen can accurately reflect the variation of rumen microorganism’s population.