CAJ | 학술논문

目的：探索七氟醚对急性肾缺血-再灌注损伤肾功能的保护作用。方法选择健康 SD 大鼠90只，随机分为三组：伪手术组、对照组及七氟醚预处理组，建立肾缺血-再灌注模型，测定各组大鼠平均动脉压（MAP）、二氧化碳分压（PCO2）、血清尿素氮（BUN）、肌酐（Cr）及超氧化物歧化酶（SOD）的变化，HE 染色观察各组大鼠肾组织病理改变。结果随着七氟醚预处理浓度的增加，大鼠 MAP 呈下降趋势，但在七氟醚浓度2％-3％的临床常用剂量范围内，大鼠的 MAP 波动于92．1±6．0 mmHg。各组肾缺血前与再灌注后，PCO2浓度无明显变化。对照组及七氟醚预处理组 BUN 和 Cr 于再灌注后12 h、24 h 均明显高于伪手术组（P ＜0．05），但七氟醚预处理组再灌注后12 h、24 h 的BUN、Cr 水平均较对照组明显下降（P ＜0．05）。七氟醚预处理组和对照组均较伪手术组血清 SOD 活力减低，但七氟醚预处理组 SOD 活力较对照组高，差异具有统计学意义（P ＜0．05）。肾脏病理观察发现伪手术组肾脏组织结构完整，对照组肾脏外髓部分组织结构严重破坏，而七氟醚预处理组肾脏组织破坏较小，肾小管组织相对完整。结论七氟醚吸入式麻醉能够有效降低 BUN、Cr 水平，保护 SOD 活力，对急性肾缺血-再灌注损伤肾功能具有一定的保护作用。
목적：탐색칠불미대급성신결혈-재관주손상신공능적보호작용。방법선택건강 SD 대서90지，수궤분위삼조：위수술조、대조조급칠불미예처리조，건립신결혈-재관주모형，측정각조대서평균동맥압（MAP）、이양화탄분압（PCO2）、혈청뇨소담（BUN）、기항（Cr）급초양화물기화매（SOD）적변화，HE 염색관찰각조대서신조직병리개변。결과수착칠불미예처리농도적증가，대서 MAP 정하강추세，단재칠불미농도2％-3％적림상상용제량범위내，대서적 MAP 파동우92．1±6．0 mmHg。각조신결혈전여재관주후，PCO2농도무명현변화。대조조급칠불미예처리조 BUN 화 Cr 우재관주후12 h、24 h 균명현고우위수술조（P ＜0．05），단칠불미예처리조재관주후12 h、24 h 적BUN、Cr 수평균교대조조명현하강（P ＜0．05）。칠불미예처리조화대조조균교위수술조혈청 SOD 활력감저，단칠불미예처리조 SOD 활력교대조조고，차이구유통계학의의（P ＜0．05）。신장병리관찰발현위수술조신장조직결구완정，대조조신장외수부분조직결구엄중파배，이칠불미예처리조신장조직파배교소，신소관조직상대완정。결론칠불미흡입식마취능구유효강저 BUN、Cr 수평，보호 SOD 활력，대급성신결혈-재관주손상신공능구유일정적보호작용。
Objective To explore the protective effects of sevoflurane on renal function in acute renal ischemia-reperfusion injury.Methods 90 healthy SD rats were randomly divided into three groups:sham-operated group,control group,sevoflurane inhalated group.Acute renal ischemia reperfusion model were established by clamping the renal pedi-cle.Mean arterial pressure(MAP),partial pressure of carbon dioxide(PCO2 ),blood urea nitrogen(BUN),creatinine (Cr)and superoxide dismutas-e(SOD)levels were determined in each groups,the renal pathological changes were ob-served by HE staining.Results With the increase in the concentration ofsevoflurance,the mean arterial pressure of the rats decreased,But the usual clinical doses in the range of sevoflurane concentration in 2%-3%,MAP is 92.1 ± 6.0 mmHg.There is no significant difference of PCO2 concentration in each group before ischemia and after reperfusion.Af-ter renal ischemia reperfusion 12 h,24 h,the BUN and Cr levels of control group and sevoflurane inhalated group were significantly higher than that in sham-operated group (P <0.05),but the BUN,Cr levels of sevoflurane inhalated group were decreased significantly compared with B group (P <0.05).Compared with sham-operated group,the serum SOD activity of control group and sevoflurane inhalated group were significantly decreased (P <0.05),But the activity of SOD of sevoflurane inhalated group was higher than control group (P < 0.05).The renal pathological structure of sham-operated group was integrity,the damage of renal outer medullary tissue was severly in control group,but the sevoflurane inhalated group kidney tubular tissue damage were lightly.Conclusion Sevoflurane inhalation anesthesia can effectively reduce BUN,Cr level,protect SOD activity,and thus,has a protective effect on renal function on acute renal ischemia/reperfusion injury.