CAJ | 학술논문

　　用农杆菌介导法将高亲和性钾离子转运体基因(HAK)和 Bar 基因转入5个优良玉米自交系7922、P138、265、238和271中，并对影响其遗传转化效率因素进行了优化。经 PCR 和 RT-PCR 检测证实获得阳性植株。除草剂涂抹实验证明 Bar 基因已经整合进玉米基因组。用不同浓度的盐溶液处理转基因植株和对照植株，发现转基因植株叶片中的K＋含量、脯氨酸和叶绿素含量均高于未转化植株，而Na＋含量低于未转化植株，表明HAK基因已经整合进玉米基因组，并通过过量表达提高了植株的耐盐性。
　　용농간균개도법장고친화성갑리자전운체기인(HAK)화 Bar 기인전입5개우량옥미자교계7922、P138、265、238화271중，병대영향기유전전화효솔인소진행료우화。경 PCR 화 RT-PCR 검측증실획득양성식주。제초제도말실험증명 Bar 기인이경정합진옥미기인조。용불동농도적염용액처리전기인식주화대조식주，발현전기인식주협편중적K＋함량、포안산화협록소함량균고우미전화식주，이Na＋함량저우미전화식주，표명HAK기인이경정합진옥미기인조，병통과과량표체제고료식주적내염성。
The HAK and Bar genes were transferred into five different inbred lines of maize,including 7922, P138,265,238 and 271,by Agrobacterium-mediated transformation. Factors influencing transformation efficiency were optimized. Transgenic plants, which are resistant to phosphinothricin (PPT), were acquired by PCR and RT-PCR. When treated with different concentration of NaCl, higer K+,proline and chlorophyll contents were observed in transgenic leaves than in wild type leaves,but Na+content in transgenic leaves was obviously lower than in wild type leaves. These results show that HAK gene is integrated into maize genome and transcribed normally. Over-expressing of HAK gene enhances the salt tolerance of transgenic maize.