CAJ | 학술논문

　　目的分离培养扩增大鼠脂肪源间充质干细胞(ADSCs)，以活体标记并鉴定其分化潜能，了解ADSCs的X连锁凋亡抑制蛋白（XIAP）基因修饰的可行性。方法无菌条件下取大鼠一侧腹股沟脂肪组织，Ⅰ型胶原酶消化法分离培养ADSCs，胰酶消化法传代扩增。检测细胞分化为脂肪细胞、软骨细胞及成骨细胞的潜能，转染XIAP表达质粒进入ADSCs，通过Western blotting等方法检测XIAP的表达能力。结果 ADSCs呈长梭形漩涡样生长，细胞流式鉴定显示CD29、CD44、CD90、CD105均呈高表达，并在特定诱导剂下分化为脂肪细胞、软骨细胞或成骨细胞。XIAP转染后显像经XIAP基因修饰的脂肪间充质干细胞在PVDF膜的相应分子质量区域出现相应的条带。结论脂肪源干细胞易于培养和传代扩增，并可活体标记，具有多向分化潜能，可作为组织工程的种子细胞。
　　목적분리배양확증대서지방원간충질간세포(ADSCs)，이활체표기병감정기분화잠능，료해ADSCs적X련쇄조망억제단백（XIAP）기인수식적가행성。방법무균조건하취대서일측복고구지방조직，Ⅰ형효원매소화법분리배양ADSCs，이매소화법전대확증。검측세포분화위지방세포、연골세포급성골세포적잠능，전염XIAP표체질립진입ADSCs，통과Western blotting등방법검측XIAP적표체능력。결과 ADSCs정장사형선와양생장，세포류식감정현시CD29、CD44、CD90、CD105균정고표체，병재특정유도제하분화위지방세포、연골세포혹성골세포。XIAP전염후현상경XIAP기인수식적지방간충질간세포재PVDF막적상응분자질량구역출현상응적조대。결론지방원간세포역우배양화전대확증，병가활체표기，구유다향분화잠능，가작위조직공정적충자세포。
Objective To investigate the feasibility of genetically modified X-linked inhibitor of apoptosis protein (XIAP) of rat adipose-derived mesenchymal stem cells (ADSCs) by isolating and cultivating rat ADSCs in vitro. Methods ADSCs were isolated from rat groin fat pads by collagenaseⅠdigestion under sterile condition. ADSCs were passaged and amplified with 10%FBS DMEM. The multi-differentiation potential of ADSCs was verified by cultivated with differentiation medium. XIAP expression plasmid was transfected into ADSCs. The anti-apoptotic ability of XIAP transduction was detect-ed by Western blotting assay. Results ADSCs were mainly spindle-shaped and whirlpool-shaped arranged. Results of flow cytometry showed that there were higher expressions of CD29, CD44, CD90 and CD105 in ADSCs, which differentiated into lipocytes, chondrocytes and osteoblasts under specific conditions. There is XIAP gene modified adipose-derived mesenchy-mal stem cells Band in the corresponding molecular mass of PVDF membrane area. Conclusion ADSCs were isolated from rat subcutaneous fat pads and were easily cultivated, passaged and amplified. ADSCs can differentiate into osteoblasts, chon-drocytes and adipocytes under specific conditions, which are better resource for being used in cell therapy and tissue engi-neering.