天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2013年
8期
752-755
,共4页
谢兆兰%周立云%王明林
謝兆蘭%週立雲%王明林
사조란%주립운%왕명림
螺杆菌,幽门%胃炎%T淋巴细胞,辅助诱导%GATA3转录因子%细胞毒素相关蛋白A%TBX21%FoxP3%Rorγt
螺桿菌,幽門%胃炎%T淋巴細胞,輔助誘導%GATA3轉錄因子%細胞毒素相關蛋白A%TBX21%FoxP3%Rorγt
라간균,유문%위염%T림파세포,보조유도%GATA3전록인자%세포독소상관단백A%TBX21%FoxP3%Rorγt
Helicobacter pylori%gastritis%T-lymphocytes,helper-inducer%GATA3 transcription factor%CagA%TBX21%FoxP3%Rorγt
目的探讨幽门螺杆菌CagA的表达对慢性胃炎患者胃黏膜Th细胞亚群分化的影响。方法80例慢性胃炎患者，ELISA法测定血清CagA抗体，根据结果将慢性胃炎患者分为CagA阳性和阴性组，均取患者胃黏膜行病理检查及PCR、Western blot检测核转录因子TBX21、GATA-3、FoxP3和Rorγt的表达水平。结果 CagA抗体阳性组和阴性组分别为52例和28例，CagA抗体阳性组炎症中、重度43例（82.7%）显著高于阴性组10例（35.7%），差异有统计学意义（χ2=17.964，P<0.05）；2组间Hp菌密度差异无统计学意义。CagA抗体阳性组TBX21、Rorγt的转录及蛋白表达水平较阴性组显著下调，而GATA-3、FoxP3的转录及蛋白表达水平较阴性组显著上调。结论慢性胃炎CagA阳性患者的胃黏膜炎症程度更重，但机体却不能有效清除Hp在胃黏膜中的定植，其原因与可能存在Th0向Th2/Treg的分化偏倚有关。
目的探討幽門螺桿菌CagA的錶達對慢性胃炎患者胃黏膜Th細胞亞群分化的影響。方法80例慢性胃炎患者，ELISA法測定血清CagA抗體，根據結果將慢性胃炎患者分為CagA暘性和陰性組，均取患者胃黏膜行病理檢查及PCR、Western blot檢測覈轉錄因子TBX21、GATA-3、FoxP3和Rorγt的錶達水平。結果 CagA抗體暘性組和陰性組分彆為52例和28例，CagA抗體暘性組炎癥中、重度43例（82.7%）顯著高于陰性組10例（35.7%），差異有統計學意義（χ2=17.964，P<0.05）；2組間Hp菌密度差異無統計學意義。CagA抗體暘性組TBX21、Rorγt的轉錄及蛋白錶達水平較陰性組顯著下調，而GATA-3、FoxP3的轉錄及蛋白錶達水平較陰性組顯著上調。結論慢性胃炎CagA暘性患者的胃黏膜炎癥程度更重，但機體卻不能有效清除Hp在胃黏膜中的定植，其原因與可能存在Th0嚮Th2/Treg的分化偏倚有關。
목적탐토유문라간균CagA적표체대만성위염환자위점막Th세포아군분화적영향。방법80례만성위염환자，ELISA법측정혈청CagA항체，근거결과장만성위염환자분위CagA양성화음성조，균취환자위점막행병리검사급PCR、Western blot검측핵전록인자TBX21、GATA-3、FoxP3화Rorγt적표체수평。결과 CagA항체양성조화음성조분별위52례화28례，CagA항체양성조염증중、중도43례（82.7%）현저고우음성조10례（35.7%），차이유통계학의의（χ2=17.964，P<0.05）；2조간Hp균밀도차이무통계학의의。CagA항체양성조TBX21、Rorγt적전록급단백표체수평교음성조현저하조，이GATA-3、FoxP3적전록급단백표체수평교음성조현저상조。결론만성위염CagA양성환자적위점막염증정도경중，단궤체각불능유효청제Hp재위점막중적정식，기원인여가능존재Th0향Th2/Treg적분화편의유관。
Objective To evaluate the influence of cytotoxin-associated gene A (CagA)to the differentiation of helper T lymphocytes in chronic gastritis. Methods Eighty patients with chronic gastritis were included in this study. The serum antibody against CagA was detected by the enzyme-linked immunosorbant assay (ELISA). Patients were divided into CagA-positive group and CagA-negative group according to the results of ELISA. Pathologic changes in gastric mucus were respectively analyzed. In addition, the expressions of nuclear transcription factors in gastric mucus including TBX 21, GATA-3, FoxP3 and Rorγt were detected by PCR and Western blot assay. Results There were 52 patients in CagA-positive group and 28 patients in CagA-negative group. The gastric inflammation was more serious in CagA-positive group than that in CagA-snegative group. There was no significant difference in Hp density between two groups. The expressions of GATA-3 and FoxP3 were much higher, while the transcription and protein expression levels of TBX21 and Rorγt were significantly lower in CagA-positive group than those in CagA-negative group. Conclusion Although the inflammation in gastric mucus was more serious in CagA-positive patients, Hp can not be effectively eliminated,which may relate to the differentiation of Th0 into Th2/Treg cells.
