CAJ | 학술논문

　　目的探讨miR-224和miR-378e在原位大肠癌癌组织和癌旁正常黏膜组织中的表达差异，并分析其临床意义。方法 miRNA表达谱芯片技术检测大肠癌癌组织和癌旁组织标本中表达差异的miRNA，运用荧光实时定量聚合酶链反应（real-time PCR）验证其结果，并分析其与临床病理资料之间的关系。结果 miRNA表达芯片分析发现，与正常黏膜组织比较，miR-224在大肠癌组织中表达显著上调，miR-378e在大肠癌组织中表达显著下调，并被real-time PCR所证实。miR-224在不同组织学类型癌组织中表达差异有统计学意义，miR-378e在不同浸润深度癌组织中表达差异有统计学意义，miR-224表达与组织学类型有关，miR-378e表达与大肠癌浸润深度有关。结论miR-224具有潜在的癌基因作用，miR-378e具有潜在的抑癌基因作用，miR-224和miR-378e可作为潜在的大肠癌分子标志物。
　　목적탐토miR-224화miR-378e재원위대장암암조직화암방정상점막조직중적표체차이，병분석기림상의의。방법 miRNA표체보심편기술검측대장암암조직화암방조직표본중표체차이적miRNA，운용형광실시정량취합매련반응（real-time PCR）험증기결과，병분석기여림상병리자료지간적관계。결과 miRNA표체심편분석발현，여정상점막조직비교，miR-224재대장암조직중표체현저상조，miR-378e재대장암조직중표체현저하조，병피real-time PCR소증실。miR-224재불동조직학류형암조직중표체차이유통계학의의，miR-378e재불동침윤심도암조직중표체차이유통계학의의，miR-224표체여조직학류형유관，miR-378e표체여대장암침윤심도유관。결론miR-224구유잠재적암기인작용，miR-378e구유잠재적억암기인작용，miR-224화miR-378e가작위잠재적대장암분자표지물。
Objective To investigate the expression and clinical significance of microRNA-224 and microRNA-378e in colorectal cancer tissues and normal mucosa adjacent to tumor lesions. Methods The gene chip technology was used to detect the different expression of miRNA in colorectal carcinoma tissues and adjacent normal tissues, which was then confirmed by real-time PCR. The relationship between the pathology and clinical data was analyzed. Results The expres-sion level of miR-224 was significantly up-regulated in tumor tissue, while miR-378e was down-regulated in tumor tissue, which was confirmed by real-time PCR. The expression of miR-224 was strongly associated with histological types, while miR-378e was strongly associated with the infiltration depth of colorectal cancer. Conclusion miR-224 is a potent tumor promoter, while miR-378e is a potent tumor suppressor. Both miR-224 and miR-378e can be used as potential colorectal cancer molecular markers.