天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2013年
9期
878-880
,共3页
糖尿病,2型%胰腺%肌,骨骼%血糖%胰岛素%大鼠,Sprague-Dawley%胰岛素受体底物1%蓬灰
糖尿病,2型%胰腺%肌,骨骼%血糖%胰島素%大鼠,Sprague-Dawley%胰島素受體底物1%蓬灰
당뇨병,2형%이선%기,골격%혈당%이도소%대서,Sprague-Dawley%이도소수체저물1%봉회
diabetes mellitus,type 2%pancreas%muscle,skeletal%blood glucose%insulin%rats,Sprague-Dawley%insu-lin receptor substance 1%Peng-Hui
目的观察不同剂量蓬灰对2型糖尿病（T2DM）大鼠胰腺及骨骼肌中胰岛素受体底物1（IRS-1）表达的影响。方法将高脂高热卡饲料喂养的大鼠给予腹腔注射小剂量链脲佐菌素（STZ）制作T2DM模型，成模后将大鼠随机分为糖尿病对照组、蓬灰低剂量组、中剂量组和高剂量组。高、中、低剂量组分别给予蓬灰150、100、50 mg/kg，另设正常对照组。蓬灰混悬液灌胃8周后，应用免疫组化学方法检测各组大鼠胰腺及骨骼肌IRS-1表达情况，结合血清血糖及胰岛素水平进行分析。结果中剂量组血糖水平低于糖尿病对照组及低剂量组（P＜0.05）；高、中、低剂量组胰岛素含量与糖尿病对照组比较差异均无统计学意义。高剂量组的胰腺和骨骼肌组织中IRS-1表达水平均低于糖尿病对照组（P＜0.05）。结论大剂量蓬灰可以降低2型糖尿病大鼠胰腺和骨骼肌组织中IRS-1的表达水平，但短期内可能不会对糖尿病大鼠血糖及血清胰岛素产生影响。
目的觀察不同劑量蓬灰對2型糖尿病（T2DM）大鼠胰腺及骨骼肌中胰島素受體底物1（IRS-1）錶達的影響。方法將高脂高熱卡飼料餵養的大鼠給予腹腔註射小劑量鏈脲佐菌素（STZ）製作T2DM模型，成模後將大鼠隨機分為糖尿病對照組、蓬灰低劑量組、中劑量組和高劑量組。高、中、低劑量組分彆給予蓬灰150、100、50 mg/kg，另設正常對照組。蓬灰混懸液灌胃8週後，應用免疫組化學方法檢測各組大鼠胰腺及骨骼肌IRS-1錶達情況，結閤血清血糖及胰島素水平進行分析。結果中劑量組血糖水平低于糖尿病對照組及低劑量組（P＜0.05）；高、中、低劑量組胰島素含量與糖尿病對照組比較差異均無統計學意義。高劑量組的胰腺和骨骼肌組織中IRS-1錶達水平均低于糖尿病對照組（P＜0.05）。結論大劑量蓬灰可以降低2型糖尿病大鼠胰腺和骨骼肌組織中IRS-1的錶達水平，但短期內可能不會對糖尿病大鼠血糖及血清胰島素產生影響。
목적관찰불동제량봉회대2형당뇨병（T2DM）대서이선급골격기중이도소수체저물1（IRS-1）표체적영향。방법장고지고열잡사료위양적대서급여복강주사소제량련뇨좌균소（STZ）제작T2DM모형，성모후장대서수궤분위당뇨병대조조、봉회저제량조、중제량조화고제량조。고、중、저제량조분별급여봉회150、100、50 mg/kg，령설정상대조조。봉회혼현액관위8주후，응용면역조화학방법검측각조대서이선급골격기IRS-1표체정황，결합혈청혈당급이도소수평진행분석。결과중제량조혈당수평저우당뇨병대조조급저제량조（P＜0.05）；고、중、저제량조이도소함량여당뇨병대조조비교차이균무통계학의의。고제량조적이선화골격기조직중IRS-1표체수평균저우당뇨병대조조（P＜0.05）。결론대제량봉회가이강저2형당뇨병대서이선화골격기조직중IRS-1적표체수평，단단기내가능불회대당뇨병대서혈당급혈청이도소산생영향。
Objective To observe the effects of different doses of Peng-Hui on expression levels of insulin receptor substance 1 (IRS-1) in type 2 diabetes (T2DM) rats. Methods T2DM rat model was established by feeding with high-fat-diet and intraperitoneal injecting streptozotocin (STZ). T2DM model rats were randomized into four groups: T2DM control group, high dose Peng-Hui (150 mg/kg) group, medium dose Peng-Hui (100 mg/kg) group and low dose Peng-Hui (50 mg/kg) group. The T2DM control group was fed with only high-fat-diet. The normal control group was fed with common diet. The expression levels of IRS-1 in pancreas and skeletal muscle tissues were detected by immunohistochemistry method after 8-week treatment, and fasting blood glucose (FBG) and insulin levels were also detected in five groups. Results FBG lev-els were significantly lower in medium dose Peng-Hui group than those of T2DM control group and low dose Peng-Hui group (P<0.05). There were no significant differences in insulin levels between high, medium and low dose Peng-Hui groups and T2DM control group (P>0.05). The expression levels of IRS-1 of pancreas and skeletal muscle tissues were significant-ly lower in high dose Peng-Hui group than that of T2DM control group (P<0.05). Conclusion The expression of IRS-1 was decreased in high dose Peng-Hui group, but no effects on FBG and plasma insulin.
