CAJ | 학술논문

　　试验对降解玉米赤霉烯酮（ZEN）的枯草芽孢杆菌（霉立解01G菌，MLJ01G）进行了体外降解活性研究；同时，研究霉立解01G菌对饲喂玉米赤霉烯酮污染日粮青年母猪外阴的影响。体外降解试验将霉立解01G菌发酵液分成上清液、菌悬液及胞内液，对玉米赤霉烯酮降解活性进行研究；动物试验将18头杜×长×大三元杂交青年母猪分为3组，每组6个重复，每个重复1头猪。对照组为正常日粮组（不含ZEN），试验组Ⅰ为霉变日粮组（含238μg/kg ZEN），试验组Ⅱ为霉变日粮（含240μg/kg ZEN）+2 kg/t霉立解（主要组分是01G菌工业化发酵干燥产物）组。试验时间为24 d，试验结果表明，霉立解01G菌降解ZEN的活性组分是存在于发酵上清液中的一种胞外酶；日粮污染240μg/kg ZEN即可导致青年母猪的外阴红肿，而在霉变日粮中添加霉立解01G菌能缓解外阴红肿症状。
　　시험대강해옥미적매희동（ZEN）적고초아포간균（매립해01G균，MLJ01G）진행료체외강해활성연구；동시，연구매립해01G균대사위옥미적매희동오염일량청년모저외음적영향。체외강해시험장매립해01G균발효액분성상청액、균현액급포내액，대옥미적매희동강해활성진행연구；동물시험장18두두×장×대삼원잡교청년모저분위3조，매조6개중복，매개중복1두저。대조조위정상일량조（불함ZEN），시험조Ⅰ위매변일량조（함238μg/kg ZEN），시험조Ⅱ위매변일량（함240μg/kg ZEN）+2 kg/t매립해（주요조분시01G균공업화발효간조산물）조。시험시간위24 d，시험결과표명，매립해01G균강해ZEN적활성조분시존재우발효상청액중적일충포외매；일량오염240μg/kg ZEN즉가도치청년모저적외음홍종，이재매변일량중첨가매립해01G균능완해외음홍종증상。
This research was conducted to study the degradation activity of a Bacillus subtilis (strain MLJ01G) on zearalenone in vitro, as well as the effect of MLJ01G on vulva of young sows fed diets contaminated with ZEN. In vitro study, the culture of MLJ01G was divided into different components including culture supernatant, bacteria cell, and cell extracts. The results suggested that the activity of ZEN degradation mainly was observed in the culture supernatant rather than in its cells or cell extracts, and the effect might be due to enzymatic reactions. In animal study, 18 young sows (Duroc × Landrace × Yorkshire) were randomly allocated to 3 treatments with 6 repli-cates of one pig per replicate, including:Control group (normal basal diet);TreatmentⅠ(a diet con-taining ZEN with a concentration of 238 μg/kg); Treatment Ⅱ(treatmentⅠwith a concentration of 240 μg/kg + 2 kg/t MLJ). The experiment lasted for 24 days. Results showed that the diets con-taining ZEN with a concentration of 238 μg/kg could cause the vulva of young sows to become red and swelling, and strain MLJ01G could relieve this symptoms.