CAJ | 학술논문

背景：脊髓损伤后的病理生理机制非常复杂，人们对此认识还很不全面、深入。目的：观察脊髓损伤动物模型中硬脊膜完整性对脑脊液内细胞因子水平的影响。 　　方法：采用钳夹压迫法建立新西兰大白兔脊髓损伤模型，随机分为无硬脊膜缺损组、硬脊膜缺损组、硬脊膜缺损复合膜修复组、硬脊膜缺损自体筋膜修复组。术后30 min、1 h、3 h、6 h、12 h、36 h采用酶联免疫吸附实验方法检测各组脑脊液中细胞因子白细胞介素6、白细胞介素10、肿瘤坏死因子α的变化。 　　结果与结论：无硬脊膜缺损组、硬脊膜缺损复合膜修复组和硬脊膜缺损自体筋膜修复组术后6h脑脊液中白细胞介素6、白细胞介素10、肿瘤坏死因子α水平均显著低于硬脊膜缺损组(P<0.05)。其余时间点4组间各因子水平差异无显著性意义(P>0.05)。说明维护脊髓损伤模型中硬脊膜的完整性可影响脑脊液中白细胞介素6、白细胞介素10、肿瘤坏死因子α水平，抑制炎症反应。
　　배경：척수손상후적병리생리궤제비상복잡，인문대차인식환흔불전면、심입。목적：관찰척수손상동물모형중경척막완정성대뇌척액내세포인자수평적영향。 　　방법：채용겸협압박법건립신서란대백토척수손상모형，수궤분위무경척막결손조、경척막결손조、경척막결손복합막수복조、경척막결손자체근막수복조。술후30 min、1 h、3 h、6 h、12 h、36 h채용매련면역흡부실험방법검측각조뇌척액중세포인자백세포개소6、백세포개소10、종류배사인자α적변화。 　　결과여결론：무경척막결손조、경척막결손복합막수복조화경척막결손자체근막수복조술후6h뇌척액중백세포개소6、백세포개소10、종류배사인자α수평균현저저우경척막결손조(P<0.05)。기여시간점4조간각인자수평차이무현저성의의(P>0.05)。설명유호척수손상모형중경척막적완정성가영향뇌척액중백세포개소6、백세포개소10、종류배사인자α수평，억제염증반응。
BACKGROUND:Pathophysiological mechanisms after spinal cord injury are very complex, so there is no compressive and in-depth understanding on it. OBJECTIVE:To study the effect of dura mater spinalis integrity on cytokine levels in the cerebrospinal fluid of animal models of spinal cord injury. METHODS:The white rabbit models of spinal cord injury were established using clamp compression method, and then the models were randomly divided into four groups:no dura mater spinalis defect group, dura mater spinalis defect group, dura mater spinalis defect composite with membrane repairing group and dura mater spinalis defect composite with autologous fascia repair group. Enzyme-linked immunosorbent assay was performed to detect the changes of levels of cytokines (interleukin-6, interleukin-10 and tumor necrosis factorα) in the cerebrospinal fluid at 30 minutes, 1, 3, 6, 12 and 36 hours after surgery. RESULTS AND CONCLUSION:The levels of interleukin-6, interleukin-10 and tumor necrosis factorαin the cerebrospinal fluid of the dura mater spinalis defect group, dura mater spinalis defect composite with membrane repairing group and dura mater spinalis defect composite with autologous fascia repair group were significantly lower than those of the no dura mater spinalis defect group at 6 hours after surgery (P<0.05). There were no significant differences in the levels of interleukin-6, interleukin-10 and tumor necrosis factorαat other time points between groups (P>0.05). The results indicate that maintaining the integrity of dura mater spinalis of the spinal cord injury model can affect the levels of interleukin-6, interleukin-10 and tumor necrosis factorαin the cerebrospinal fluid, thus inhibiting the inflammatory response.