CAJ | 학술논문

背景：研究证明利用电刺激外周神经纤维可恢复一些因失去中枢神经控制的肌肉的功能。 　　目的：验证双电极1 mm较近距离下双向方波脉冲实现神经选择性兴奋的正确性，并基于此实现神经的选择性兴奋。 　　方法：成年Wistar大鼠8只，麻醉后暴露大鼠坐骨神经，将电极小心放于坐骨神经干，建立神经选择性刺激模型。实验用电极为自制Cuff双极性电极，刺激器采用的是Grass S88刺激器和AWG2005任意波形信号发生器。采取双电极双向刺激方式，两个电极之间距离为1 mm，刺激波形选用脉宽为0.2 ms的对称双向脉冲，其输出脉冲的幅度、脉宽和延时均可调。调节刺激强度，研究双电极双向刺激下神经兴奋性的规律，以此实现神经的选择性兴奋，并利用“碰撞法”原理验证利用双电极双向刺激方法实现神经选择性兴奋的可行性。结果与结论：实验过程中神经动作电位的变化将经 P511放大器放大后接入示波器显示，双电极刺激波形为脉宽为0.2 ms的对称双向脉冲。随着刺激幅度的增大，实现神经的选择性兴奋。说明用距离很近(1 mm)的双电极双向对称脉冲的方法实现了神经的选择性兴奋，并利用“碰撞法”原理证实了此种方法的有效性和可行性。
　　배경：연구증명이용전자격외주신경섬유가회복일사인실거중추신경공제적기육적공능。 　　목적：험증쌍전겁1 mm교근거리하쌍향방파맥충실현신경선택성흥강적정학성，병기우차실현신경적선택성흥강。 　　방법：성년Wistar대서8지，마취후폭로대서좌골신경，장전겁소심방우좌골신경간，건립신경선택성자격모형。실험용전겁위자제Cuff쌍겁성전겁，자격기채용적시Grass S88자격기화AWG2005임의파형신호발생기。채취쌍전겁쌍향자격방식，량개전겁지간거리위1 mm，자격파형선용맥관위0.2 ms적대칭쌍향맥충，기수출맥충적폭도、맥관화연시균가조。조절자격강도，연구쌍전겁쌍향자격하신경흥강성적규률，이차실현신경적선택성흥강，병이용“팽당법”원리험증이용쌍전겁쌍향자격방법실현신경선택성흥강적가행성。결과여결론：실험과정중신경동작전위적변화장경 P511방대기방대후접입시파기현시，쌍전겁자격파형위맥관위0.2 ms적대칭쌍향맥충。수착자격폭도적증대，실현신경적선택성흥강。설명용거리흔근(1 mm)적쌍전겁쌍향대칭맥충적방법실현료신경적선택성흥강，병이용“팽당법”원리증실료차충방법적유효성화가행성。
BACKGROUND:Studies have shown that the application of electrical stimulation of peripheral nerve fibers can recover partial muscle functions due to the loss of central nervous control. OBJECTIVE:To verify the feasibility of selective nerve excitatory effectively used the symmetric biphasic pulses under the bipolar electrodes stimulation of 1 mm. METHODS:Eight adult Wistar rats were selected to expose the sciatic nerves after anesthesia and then the electrodes were placed on the sciatic nerves careful y to establish the model of selective nerve stimulation. Experimental electrode was homemade Cuff bipolar electrode, and the electrode stimulators were Grass S88 stimulator and AWG2005 arbitrary waveform signal generator. The two-way dual-electrode stimulation was used. The distance between two electrodes was 1 mm, and the stimulation waveform was symmetric biphasic pulse with the width of 0.2 ms. The output pulse amplitude, pulse width and delay could be adjusted. The stimulation intensity was adjusted, and the law of nerve excitability was detected under two-way dual-electrode stimulation, in order to achieve selective nerve excitability, and the feasibility of two-way dual-electrode stimulation to achieve selective nerve excitatory was verified with“col ision”method. RESULTS AND CONCLUSION:The change of nerve action potential was amplified by P511 amplifier and then linked into oscil oscope for displaying, and dual-electrode stimulation waveform was the symmetric biphasic pulse with the width of 0.2 ms. With the increasing of stimulus amplitude, we achieved the selective nerve excitatory. The results indicate the selective nerve excitatory can be achieved with the closed (1 mm) dual-electrode symmetric pulse, and the feasibility and effectiveness of this method can be verified with“col ision”principle.