中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
33期
5909-5916
,共8页
上官雅娴%卫小春%李凯%卫建平
上官雅嫻%衛小春%李凱%衛建平
상관아한%위소춘%리개%위건평
组织构建%软骨组织构建%滑膜炎%膝关节%滑膜损伤%基质金属蛋白酶%长距离%跑步运动%免疫组织化学%国家自然科学基金
組織構建%軟骨組織構建%滑膜炎%膝關節%滑膜損傷%基質金屬蛋白酶%長距離%跑步運動%免疫組織化學%國傢自然科學基金
조직구건%연골조직구건%활막염%슬관절%활막손상%기질금속단백매%장거리%포보운동%면역조직화학%국가자연과학기금
tissue construction%cartilage tissue construction%synovitis%knee joint%synovial injury%matrix metal oproteinase%long-distance%running exercise%immunohistochemistry%National Natural Science Foundation of China
背景：很多研究表明基质金属蛋白酶1，3，9和13对关节软骨的退变存在影响，但是针对关节滑膜进行的专项研究相对较少。<br> 目的：观察长距离跑步运动对基质金属蛋白酶1，3，9以及基质金属蛋白酶13在滑膜上的表达的影响。<br> 方法：Wistar雄性大鼠15只随机分为3组：对照组、平板组和上坡组。对照组普通笼养；平板组每天在平板0°的水平面跑步机上以1 km/h，运动1 h，持续45 d；上坡组先在平板0°跑步机上以1 km/h，运动1 h，持续15 d，然后在上坡+20°的跑步机上每天以1 km/h，运动1 h，持续30 d。造成不同程度的膝关节滑膜损伤模型，造模成功后取双后肢膝关节，进行石蜡包埋，矢状面整体切片，而后进行苏木精-伊红和免疫组织化学染色，观察并分析实验结果。<br> 结果与结论：长距离跑步运动后，平板组和上坡组的滑膜组织中基质金属蛋白酶1的表达都比对照组增高(P <0.05)，但平板组和上坡组之间差异无显著性意义(P >0.05)；而3组基质金属蛋白酶3的表达并无明显变化(P>0.05)；基质金属蛋白酶9和基质金属蛋白酶13在滑膜组织中的表达呈梯度递增状态(P<0.05)，对照组表达最低，平板组有所升高，上坡组的表达最高。说明长距离跑步运动可通过改变基质金属蛋白酶的表达而影响大鼠膝关节滑膜组织的正常生理结构。
揹景：很多研究錶明基質金屬蛋白酶1，3，9和13對關節軟骨的退變存在影響，但是針對關節滑膜進行的專項研究相對較少。<br> 目的：觀察長距離跑步運動對基質金屬蛋白酶1，3，9以及基質金屬蛋白酶13在滑膜上的錶達的影響。<br> 方法：Wistar雄性大鼠15隻隨機分為3組：對照組、平闆組和上坡組。對照組普通籠養；平闆組每天在平闆0°的水平麵跑步機上以1 km/h，運動1 h，持續45 d；上坡組先在平闆0°跑步機上以1 km/h，運動1 h，持續15 d，然後在上坡+20°的跑步機上每天以1 km/h，運動1 h，持續30 d。造成不同程度的膝關節滑膜損傷模型，造模成功後取雙後肢膝關節，進行石蠟包埋，矢狀麵整體切片，而後進行囌木精-伊紅和免疫組織化學染色，觀察併分析實驗結果。<br> 結果與結論：長距離跑步運動後，平闆組和上坡組的滑膜組織中基質金屬蛋白酶1的錶達都比對照組增高(P <0.05)，但平闆組和上坡組之間差異無顯著性意義(P >0.05)；而3組基質金屬蛋白酶3的錶達併無明顯變化(P>0.05)；基質金屬蛋白酶9和基質金屬蛋白酶13在滑膜組織中的錶達呈梯度遞增狀態(P<0.05)，對照組錶達最低，平闆組有所升高，上坡組的錶達最高。說明長距離跑步運動可通過改變基質金屬蛋白酶的錶達而影響大鼠膝關節滑膜組織的正常生理結構。
배경：흔다연구표명기질금속단백매1，3，9화13대관절연골적퇴변존재영향，단시침대관절활막진행적전항연구상대교소。<br> 목적：관찰장거리포보운동대기질금속단백매1，3，9이급기질금속단백매13재활막상적표체적영향。<br> 방법：Wistar웅성대서15지수궤분위3조：대조조、평판조화상파조。대조조보통롱양；평판조매천재평판0°적수평면포보궤상이1 km/h，운동1 h，지속45 d；상파조선재평판0°포보궤상이1 km/h，운동1 h，지속15 d，연후재상파+20°적포보궤상매천이1 km/h，운동1 h，지속30 d。조성불동정도적슬관절활막손상모형，조모성공후취쌍후지슬관절，진행석사포매，시상면정체절편，이후진행소목정-이홍화면역조직화학염색，관찰병분석실험결과。<br> 결과여결론：장거리포보운동후，평판조화상파조적활막조직중기질금속단백매1적표체도비대조조증고(P <0.05)，단평판조화상파조지간차이무현저성의의(P >0.05)；이3조기질금속단백매3적표체병무명현변화(P>0.05)；기질금속단백매9화기질금속단백매13재활막조직중적표체정제도체증상태(P<0.05)，대조조표체최저，평판조유소승고，상파조적표체최고。설명장거리포보운동가통과개변기질금속단백매적표체이영향대서슬관절활막조직적정상생리결구。
BACKGROUND:Many studies have shown that matrix metal oproteinases 1, 3, 9 and 13 play an important role in articular cartilage degeneration and destruction, but there is less special research on the articular synovium. OBJECTIVE:To observe the effect of long-distance running on the expressions of matrix metal oproteinases 1, 3, 9 and 13 in the synovium. <br> METHODS:Fifteen male Wistar rats were divided into three groups:control group, tablet group and uphil group. Rats in the control group received ordinary captivity;rats in the tablet group ran on the horizontal treadmil (0°) at the speed of 1 km/h for 1 hour daily, and lasted for 45 days;rats in the uphil group daily ran on the horizontal <br> treadmil (0°) at the speed of 1 km/h for 1 hour, and lasted for 15 days, and then the rats ran on the uphil treadmil (+20°) at the speed of 1 km/h for 1 hour daily and lasted for 30 days. The knee joint synovium injury models with varying degrees were established. The dual hind knee joints were obtained after modeling for paraffin-embedded. Then the overal sagittal slices were obtained for hematoxylin-eosin staining and immunohistochemical staining, and the experimental results were observed and analyzed. <br> RESULTS AND CONCLUSION:After long-distance running, the expression of matrix metal oproteinases 1 in synovium of the tablet group and uphil group was increased when compared with that of the control group (P<0.05), but there was no significant difference between tablet group and uphil group (P>0.05). There was no significant difference in matrix metal oproteinases 3 expression (P>0.05). The expressions of matrix metal oproteinase 9 and <br> matrix metal oproteinase 13 in synovium were in gradient increasing state (P<0.05), which were lowest in the control group, increased in the tablet group and highest in the uphil group. The results indicate that long-distance running <br> exercise can influence the normal physiological structure of rat knee joint synovium by changing the expression of matrix metal oproteinases.
