中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
33期
5901-5908
,共8页
魏立%江莉婷%周琦%朱雅萍%高益鸣
魏立%江莉婷%週琦%硃雅萍%高益鳴
위립%강리정%주기%주아평%고익명
组织构建%软骨组织构建%胰岛素样生长因子1%下颌骨髁突%软骨细胞%细胞凋亡%细胞增殖%Bax%Bcl-2%省级基金
組織構建%軟骨組織構建%胰島素樣生長因子1%下頜骨髁突%軟骨細胞%細胞凋亡%細胞增殖%Bax%Bcl-2%省級基金
조직구건%연골조직구건%이도소양생장인자1%하합골과돌%연골세포%세포조망%세포증식%Bax%Bcl-2%성급기금
tissue construction%cartilage tissue construction%insulin-like growth factor 1%mandibular condyle%chondrocytes%cel apoptosis%cel proliferation%Bax%Bcl-2%provincial grants-supported paper
背景:胰岛素样生长因子1参与了髁突软骨生长与改建,是软骨发育关键因子。<br> 目的:探索胰岛素样生长因子1对体外培养大鼠髁突软骨细胞凋亡的作用,以及对凋亡相关因子Bcl-2和Bax mRNA及蛋白表达变化的影响。<br> 方法:体外培养并鉴定出生后1,28 d大鼠髁突软骨细胞后,将每个年龄组的髁突软骨细胞分别分为实验组和对照组。饥饿培养24 h后,实验组加入100μg/L重组大鼠胰岛素样生长因子1细胞因子孵育48 h,对照组正常培养。<br> 结果与结论:与对照组相比,实验组加入重组胰岛素样生长因子1后,髁突软骨细胞数量增多,增殖速度显著增加(P<0.05)。实时PCR和Western blot检测显示,加入重组胰岛素样生长因子1培养48 h后,各组髁突软骨细胞中bcl-2 mRNA和蛋白表达增加,bax mRNA和蛋白表达减少(P <0.05)。提示胰岛素样生长因子1可以促进新出生及青春期大鼠髁突软骨细胞增殖,抑制其凋亡,并可能通过Bcl-2和Bax介导抑制凋亡。
揹景:胰島素樣生長因子1參與瞭髁突軟骨生長與改建,是軟骨髮育關鍵因子。<br> 目的:探索胰島素樣生長因子1對體外培養大鼠髁突軟骨細胞凋亡的作用,以及對凋亡相關因子Bcl-2和Bax mRNA及蛋白錶達變化的影響。<br> 方法:體外培養併鑒定齣生後1,28 d大鼠髁突軟骨細胞後,將每箇年齡組的髁突軟骨細胞分彆分為實驗組和對照組。饑餓培養24 h後,實驗組加入100μg/L重組大鼠胰島素樣生長因子1細胞因子孵育48 h,對照組正常培養。<br> 結果與結論:與對照組相比,實驗組加入重組胰島素樣生長因子1後,髁突軟骨細胞數量增多,增殖速度顯著增加(P<0.05)。實時PCR和Western blot檢測顯示,加入重組胰島素樣生長因子1培養48 h後,各組髁突軟骨細胞中bcl-2 mRNA和蛋白錶達增加,bax mRNA和蛋白錶達減少(P <0.05)。提示胰島素樣生長因子1可以促進新齣生及青春期大鼠髁突軟骨細胞增殖,抑製其凋亡,併可能通過Bcl-2和Bax介導抑製凋亡。
배경:이도소양생장인자1삼여료과돌연골생장여개건,시연골발육관건인자。<br> 목적:탐색이도소양생장인자1대체외배양대서과돌연골세포조망적작용,이급대조망상관인자Bcl-2화Bax mRNA급단백표체변화적영향。<br> 방법:체외배양병감정출생후1,28 d대서과돌연골세포후,장매개년령조적과돌연골세포분별분위실험조화대조조。기아배양24 h후,실험조가입100μg/L중조대서이도소양생장인자1세포인자부육48 h,대조조정상배양。<br> 결과여결론:여대조조상비,실험조가입중조이도소양생장인자1후,과돌연골세포수량증다,증식속도현저증가(P<0.05)。실시PCR화Western blot검측현시,가입중조이도소양생장인자1배양48 h후,각조과돌연골세포중bcl-2 mRNA화단백표체증가,bax mRNA화단백표체감소(P <0.05)。제시이도소양생장인자1가이촉진신출생급청춘기대서과돌연골세포증식,억제기조망,병가능통과Bcl-2화Bax개도억제조망。
BACKGROUND:Insulin-like growth factor 1 is the key factor during cartilage development, which is involved in the growth and reconstruction of condylar cartilage. <br> OBJECTIVE:To study the effect of insulin-like growth factor 1 on cel apoptosis and the apopotosis-associated factors of Bcl-2, Bax mRNA and protein expressions of rat condylar chondrocytes. <br> METHODS:The 1-day-old and 28-day-old rat condylar chondrocytes were cultured and identified in vitro. The condylar chondrocytes with different ages were divided into experimental group and control group. After being starved for 24 hours, chondrocytes in the experimental group were incubated with 100μg/L recombined rat insulin-like growth factor 1 for 48 hours, while the chondrocytes in the control group were incubated normal y. RESULTS AND CONCLUSION:Compared with the control group, after being incubated with recombined <br> insulin-like growth factor 1, the number of condylar chondrocytes was increased with high speed proliferation (P<0.05). Real-time RCR and western blot analysis revealed that the expression levels of Bcl-2 mRNA and protein were increased after added with recombined rat insulin-like growth factor 1, while the expression levels of Bax and protein were decreased (P<0.05). The results indicate that insulin-like growth factor 1 can promote the <br> proliferation and reduce cel apoptosis of newborn and adolescent rat condylar chondrocytes, which may be mediated by Bcl-2 and Bax.