CAJ | 학술논문

背景：目前体外循环肝素涂层管路应用较广，但是价格昂贵，限制了其广泛应用。 　　目的：将多醛基海藻酸钠涂层到医用聚氯乙烯体外循环管路，筛选出最佳涂层条件和所制备管路的稳定性及抗凝性能。 　　方法：利用高碘酸钠氧化海藻酸钠，制备高碘酸钠和海藻酸钠摩尔比分别为1∶8、1∶10和1∶12的多醛基氧化海藻酸钠，采用化学结合的方法将不同氧化度海藻酸钠固定到医用聚氯乙烯体外循环管路表面，筛选出最佳氧化度。以硫酸浓度、聚乙烯亚胺浓度、氧化海藻酸钠浓度、氧化海藻酸钠pH值和温度为因素，采用正交实验筛选最佳涂层条件，评价所制备管路氧化海藻酸钠固定的稳定性及抗凝血性能，并与空白对照组和肝素涂层组进行比较和分析。 　　结果与结论：氧化海藻酸钠最佳氧化度为高碘酸钠和海藻酸钠摩尔比为1∶10。筛选出的最佳涂层条件为50%浓硫酸、0.05%聚乙烯亚胺、反应溶液pH值为3.5、反应温度为40℃和氧化海藻酸钠质量浓度为2 g/L。氧化海藻酸钠涂层管路组脱落率与肝素涂层组有相似的结果，抗凝血性能稍劣于肝素涂层组(P<0.05)，但显著优于空白对照组。表明氧化海藻酸钠涂层体外循环管路具有良好的抗凝血性能和较好的稳定性。
　　배경：목전체외순배간소도층관로응용교엄，단시개격앙귀，한제료기엄범응용。 　　목적：장다철기해조산납도층도의용취록을희체외순배관로，사선출최가도층조건화소제비관로적은정성급항응성능。 　　방법：이용고전산납양화해조산납，제비고전산납화해조산납마이비분별위1∶8、1∶10화1∶12적다철기양화해조산납，채용화학결합적방법장불동양화도해조산납고정도의용취록을희체외순배관로표면，사선출최가양화도。이류산농도、취을희아알농도、양화해조산납농도、양화해조산납pH치화온도위인소，채용정교실험사선최가도층조건，평개소제비관로양화해조산납고정적은정성급항응혈성능，병여공백대조조화간소도층조진행비교화분석。 　　결과여결론：양화해조산납최가양화도위고전산납화해조산납마이비위1∶10。사선출적최가도층조건위50%농류산、0.05%취을희아알、반응용액pH치위3.5、반응온도위40℃화양화해조산납질량농도위2 g/L。양화해조산납도층관로조탈락솔여간소도층조유상사적결과，항응혈성능초렬우간소도층조(P<0.05)，단현저우우공백대조조。표명양화해조산납도층체외순배관로구유량호적항응혈성능화교호적은정성。
BACKGROUND:At present, a heparin-coated extracorporeal circulation pipe is used widely, but the price is expensive and limits its wide application. OBJECTIVE:To screen the optimal coating condition of oxidated sodium alginate as a coating material to coat the medical polyvinyl chloride pipe and to evaluate the anticoagulant properties and the stability of the coated pipe. METHODS:Oxidized sodium alginate was prepared with sodium periodate, and the mole ratio of sodium periodate and sodium alginate was 1:8, 1:10 and 1:12, respectively. Oxidized sodium alginate with different degree of oxidation was coated on the surface of medical polyvinyl chloride pipes by chemical methods, then to select the best degree of oxidation. The optimal coating condition was screened through an orthogonal experiment based on concentrations of sulfuric acid, polyethyleneimine and oxidized sodium alginate, and pH value and temperature of oxidized sodium alginate. Additional y, the anticoagulant properties and expulsion rates of the prepared pipes were evaluated in comparison with the blank control group and heparin-coated group. RESULTS AND CONCLUSION:The optimal oxidation degree for oxidized sodium alginate was 1:10 for the mole ratio of sodium periodate and sodium alginate. The best coating conditions were 50%concentrated sulfuric acid, 0.05%polyethyleneimine, 40 ℃ of reaction temperature, 2 g/L oxidized sodium alginate, and pH value=3.5. The oxidized sodium alginate coating group had a similar trend compared with heparin-coated group in the expulsion rate. The anticoagulant properties of oxidized sodium alginate coating group were little weaker than those of heparin-coated group (P<0.05), but significantly better than those of the blank control group. The oxidized sodium alginate-coated extracorporeal circulation pipe has a good anticoagulant property and stability.