CAJ | 학술논문

背景：近年来，羧甲基壳聚糖的体内降解已成为牙周组织工程和材料学领域中的研究热点，但在涉及羧甲基壳聚糖酶解的文献中，尚未找到关于牙周致病菌能够产酶降解羧甲基壳聚糖的报道。 　　目的：研究几种常见牙周致病菌对羧甲基壳聚糖的降解作用。 　　方法：制备质量分数1%和2%的羧甲基壳聚糖厌氧微生物培养基。采用透明圈法，分别挑取牙龈卟啉单胞菌、伴放线放线杆菌和黏性放线菌菌落划线接种于羧甲基壳聚糖厌氧微生物培养基中，置于厌氧培养袋中37℃恒温培养，观察结果，如果菌落周围出现明显的透明圈，即为阳性，说明细菌能够分解羧甲基壳聚糖，反之则为阴性，并测量菌落直径。 　　结果与结论：牙龈卟啉单胞菌在1%羧甲基壳聚糖厌氧微生物培养基上生长良好，培养18 d后菌落周围出现明显的透明圈；在2%羧甲基壳聚糖培养基上，牙龈卟啉单胞菌的生长受到抑制，未形成形态规则的菌落；伴放线放线杆菌和黏性放线菌在两种质量分数羧甲基壳聚糖厌氧微生物培养基中，菌落周围均未出现透明圈，培养2周后菌落干枯失去活性。提示牙龈卟啉单胞菌可降解较低质量分数的羧甲基壳聚糖，伴放线放线杆菌和黏性放线菌均不能降解羧甲基壳聚糖。
　　배경：근년래，최갑기각취당적체내강해이성위아주조직공정화재료학영역중적연구열점，단재섭급최갑기각취당매해적문헌중，상미조도관우아주치병균능구산매강해최갑기각취당적보도。 　　목적：연구궤충상견아주치병균대최갑기각취당적강해작용。 　　방법：제비질량분수1%화2%적최갑기각취당염양미생물배양기。채용투명권법，분별도취아간계람단포균、반방선방선간균화점성방선균균락화선접충우최갑기각취당염양미생물배양기중，치우염양배양대중37℃항온배양，관찰결과，여과균락주위출현명현적투명권，즉위양성，설명세균능구분해최갑기각취당，반지칙위음성，병측량균락직경。 　　결과여결론：아간계람단포균재1%최갑기각취당염양미생물배양기상생장량호，배양18 d후균락주위출현명현적투명권；재2%최갑기각취당배양기상，아간계람단포균적생장수도억제，미형성형태규칙적균락；반방선방선간균화점성방선균재량충질량분수최갑기각취당염양미생물배양기중，균락주위균미출현투명권，배양2주후균락간고실거활성。제시아간계람단포균가강해교저질량분수적최갑기각취당，반방선방선간균화점성방선균균불능강해최갑기각취당。
BACKGROUND:In recent years, the degradation in vivo of carboxymethyl chitosan has become a research focus in the fields of periodontal tissue engineering and materials science. However, in the literature involving carboxymethyl chitosan enzymatic degradation, there is no report regarding whether common periodontal pathogens could degradate carboxymethyl chitosan. OBJECTIVE:To study the degradation of carboxymethyl chitosan by several common periodontal pathogens. METHODS:1%and 2%carboxymethyl chitosan anaerobic microbiological culture media were prepared. The colonies of Porphyromonas gingivalis, Actinobacil us actinomycetemcomitan and Actinomyces viscosus were picked up streaking onto the carboxymethyl chitosan medium, respectively, which was then incubated in anaerobic culture bag at 37 ℃. If the see-through circle appeared around the colonies, the result was positive, indicating that the bacteria can degradate carboxymethyl chitosan;on the contrary, it was negative. Afterwards, the diameter of the colonies was measured. RESULTS AND CONCLUSION:It was observed that the colonies of Porphyromonas gingivalis grew wel in the 1%carboxymethyl chitosan medium and the see-through circles appeared around the colonies after incubation for 18 days;meanwhile, the medium containing 2%carboxymethyl chitosan inhibited the growth of Porphyromonas gingivalis and the standard morphological colonies did not form wel . The see-through circle did not appear around the colonies of Actinobacil us actinomycetemcomitan and Actinomyces viscosus in the two concentrations of carboxymethyl chitosan media and the colonies were dried-up and lost activity after 2-week incubation. The results indicated that the lower concentration of carboxymethyl chitosan could induce Porphyromonas gingivalis to produce chitosanase and degradate carboxymethyl chitosan. Neither Actinobacil us actinomycetemcomitan nor Actinomyces viscosus could degradate carboxymethyl chitosan.