CAJ | 학술논문

背景：聚氨酯具有良好的机械物理特性，被广泛应用于医学临床和实验研究，但聚氨酯表面的疏水性使其与组织细胞相容性不理想，从而在一定程度上制约了它作为组织工程中生物材料支架的应用。 　　目的：观察聚氨酯平面膜在接枝丝素蛋白和明胶蛋白后的亲水性及其与人下咽细胞的相容性。 　　方法：采用接触角测试仪分析丝素蛋白或明胶蛋白改性前后聚氨酯平面膜的亲水性能。将体外培养原代人下咽成纤维细胞分别接种于聚氨酯平面膜、丝素蛋白改性聚氨酯平面膜、明胶蛋白改性聚氨酯平面膜及组织培养平板中，采用细胞计数法、细胞形态观察法比较改性前后聚氨酯平面膜的细胞相容性变化。 　　结果与结论：与改性前比较，丝素蛋白或明胶蛋白改性后的聚氨酯平面膜亲水性显著增高(P<0.01)，并且丝素蛋白改性聚氨酯平面膜的亲水性高于明胶蛋白改性的聚氨酯平面膜(P<0.01)。在组织培养平板中黏附的细胞数量最多，增殖最快；在丝素蛋白或明胶蛋白改性聚氨酯平面膜中黏附的人下咽成纤维细胞均多于聚氨酯平面膜上的黏附细胞数量，且以丝素蛋白改性聚氨酯平面膜上的细胞较多。表明丝素蛋白或明胶蛋白改性聚氨酯平面膜的亲水性及细胞相容性均有所提高。
　　배경：취안지구유량호적궤계물리특성，피엄범응용우의학림상화실험연구，단취안지표면적소수성사기여조직세포상용성불이상，종이재일정정도상제약료타작위조직공정중생물재료지가적응용。 　　목적：관찰취안지평면막재접지사소단백화명효단백후적친수성급기여인하인세포적상용성。 　　방법：채용접촉각측시의분석사소단백혹명효단백개성전후취안지평면막적친수성능。장체외배양원대인하인성섬유세포분별접충우취안지평면막、사소단백개성취안지평면막、명효단백개성취안지평면막급조직배양평판중，채용세포계수법、세포형태관찰법비교개성전후취안지평면막적세포상용성변화。 　　결과여결론：여개성전비교，사소단백혹명효단백개성후적취안지평면막친수성현저증고(P<0.01)，병차사소단백개성취안지평면막적친수성고우명효단백개성적취안지평면막(P<0.01)。재조직배양평판중점부적세포수량최다，증식최쾌；재사소단백혹명효단백개성취안지평면막중점부적인하인성섬유세포균다우취안지평면막상적점부세포수량，차이사소단백개성취안지평면막상적세포교다。표명사소단백혹명효단백개성취안지평면막적친수성급세포상용성균유소제고。
BACKGROUND:Polyurethane has good mechanical and physical characteristics and is extensively used in clinical and experimental studies, but its hydrophobicity and histocompatibility are not ideal, which limits its use in tissue engineering as a biomaterial scaffold to some extents. OBJECTIVE:To observe the hydrophilicity of polyurethane membrane grafted with silk fibroin and glutin and its compatibility with human hypopharyngeal cel s. METHODS:The changes in hydrophilicity of polyurethane membrane grafted with silk fibroin and glutin were detected by contact angle measurements. Human hypopharyngeal fibroblasts were cultured in vitro on polyurethane membrane, silk fibroin-polyurethane membrane, glutin-polyurethane membrane and tissue culture plate. Cel compatibility was compared using cytometry and cel morphology obsevation. RESULTS AND CONCLUSION:Hydrophilicity of silk fibroin-or glutin-polyurethane membranes significantly increased (P<0.01). The hydrophilicity of silk fibroin-polyurethane membrane was higher than that of glutin-polyurethane membrane (P<0.01). The number of cel s on the tissue culture plate was the most. The number of human hypopharyngeal fibroblasts on the silk fibroin-or glutin-polyurethane membrane was higher than that on the polyurethane membrane, especial y on the silk fibroin-polyurethane membrane. These suggested that hydrophilicity and cel compatibility of silk fibroin-or glutin-polyurethane membrane were elevated.