CAJ | 학술논문

背景：前期研究制备的多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜厚度较厚，且孔洞不均匀。 　　目的：制备厚度薄且分布均匀的多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜，检测其细胞相容性及对细胞分化的作用。 　　方法：通过相分离法制备厚度薄且分布均匀的无孔及多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜，检测其厚度及失重率。将人骨髓间充质干细胞分别与无孔及多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜共培养7 d，扫描电镜观察复合膜的超微结构，流式细胞术分析复合膜上骨髓间充质干细胞的表面标记物。 　　结果与结论：多孔和无孔复合膜的厚度分别为(0.041±0.005) mm和(0.058±0.004) mm，24 h失重率分别为19.93%和7.64%。无孔复合膜上的偏磷酸钙粒子分布均匀，细胞完全铺展，呈梭形；多孔复合膜上的偏磷酸钙粒子分布均匀，孔径分布也均匀，孔径2-8μm，细胞全完铺展，呈多边形，有多个触角，部分细胞触角进入支架内部。无孔与多孔复合膜上的细胞均表达CD105、CD90、CD44、CD29及CD73，组间细胞阳性率差异无显著性意义。实验制备的聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜具有良好的细胞相容性，但无促细胞分化作用。
　　배경：전기연구제비적다공취3-간기정산-4-간기정산/편린산개복합막후도교후，차공동불균균。 　　목적：제비후도박차분포균균적다공취3-간기정산-4-간기정산/편린산개복합막，검측기세포상용성급대세포분화적작용。 　　방법：통과상분리법제비후도박차분포균균적무공급다공취3-간기정산-4-간기정산/편린산개복합막，검측기후도급실중솔。장인골수간충질간세포분별여무공급다공취3-간기정산-4-간기정산/편린산개복합막공배양7 d，소묘전경관찰복합막적초미결구，류식세포술분석복합막상골수간충질간세포적표면표기물。 　　결과여결론：다공화무공복합막적후도분별위(0.041±0.005) mm화(0.058±0.004) mm，24 h실중솔분별위19.93%화7.64%。무공복합막상적편린산개입자분포균균，세포완전포전，정사형；다공복합막상적편린산개입자분포균균，공경분포야균균，공경2-8μm，세포전완포전，정다변형，유다개촉각，부분세포촉각진입지가내부。무공여다공복합막상적세포균표체CD105、CD90、CD44、CD29급CD73，조간세포양성솔차이무현저성의의。실험제비적취3-간기정산-4-간기정산/편린산개복합막구유량호적세포상용성，단무촉세포분화작용。
BACKGROUND:Porous poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate composite membranes prepared previously is too thick and uneven in holes. OBJECTIVE:To prepare the thin even porous poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate composite membrane, and to evaluate the cytocompatibility and differentiation capacity. METHODS:Porous and nonporous, thin and even poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate composite membranes were prepared by phase separation method. Its thickness and weight loss rate were determined. Human bone marrow mesenchymal stem cel s were cocultured with porous and nonporous poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate composite membranes for 7 days. Ultrastructure of composite membranes was observed under the scanning electron microscopy. Surface markers of the bone marrow mesenchymal stem cel s on the composite membranes were analyzed using flow cytometry. RESULTS AND CONCLUSION:The thickness of the porous and nonporous composite membranes was (0.041 ± 0.005) mm and (0.058±0.004) mm. Weight loss rates of porous and nonporous composite membranes were respectively 19.93%and 7.64%at 24 hours. Calcium metaphosphate particles were evenly distributed in porous and nonporous composite membrane. Cel s spread entirely, showing spindle shape. Calcium metaphosphate particles were evenly distributed in porous composite membrane. Pore in porous composite membranes was also uniformly distributed, and pore size was about 2-8μm. Cel s spread entirely, showing polygonal shape with multiple tentacles. The tentacles of some cel s entered into the scaffold. CD105, CD90, CD44, CD29 and CD73 expression was detected in porous and nonporous composite membranes. There was no significant difference in cel-positive rate. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate composite membranes prepared in this study has good biocompatibility and could not promote cel differentiation.