CAJ | 학술논문

背景：异种生物骨经适当理化方法处理及高温锻烧后能够形成具有与人骨结构相近的天然网状孔隙结构的陶瓷样异种骨，具有三维立体孔隙-网架结构，有利于种子细胞的黏附、增殖。 　　目的：观察以高温煅烧骨及骨髓间充质干细胞复合构建形成的组织工程化骨修复牙槽骨缺损的可行性。 　　方法：以羊骨髓间充质干细胞为种子细胞，高温煅烧骨为支架材料，复合构建形成组织工程化骨。全麻下分批次拔除羊双侧下颌第一前磨牙，去除远中根与第二前磨牙近中根间牙槽嵴间隔，形成5 mm×5 mm×5 mm骨缺损区域。将12只实验羊随机等分为组织工程化骨组和单纯煅烧骨组，分别在左侧下颌术区放组织工程化骨和单纯煅烧骨，所有动物右侧均作为空白对照组。 　　结果与结论：高温煅烧骨呈白垩色，保留了天然松质骨的多孔状空间。孔隙率(66.10±1.32)%，孔径范围137.44-538.72μm。干细胞接种到煅烧骨24 h后可见细胞黏附支架上，7 d后分泌胞外基质，细胞与基质分界不清。X 射线可见组织工程化骨组和单纯煅烧骨组植入材料包埋在术区，煅烧骨边缘有一圈低密度阴影。苏木精-伊红染色实验侧骨小梁形成，对照侧无明显骨生成。提示应用骨髓间充质干细胞复合煅烧骨构建的组织工程化骨可较好的修复牙槽骨缺损，是修复小范围骨缺损理想的种子细胞和支架材料。
　　배경：이충생물골경괄당이화방법처리급고온단소후능구형성구유여인골결구상근적천연망상공극결구적도자양이충골，구유삼유입체공극-망가결구，유리우충자세포적점부、증식。 　　목적：관찰이고온단소골급골수간충질간세포복합구건형성적조직공정화골수복아조골결손적가행성。 　　방법：이양골수간충질간세포위충자세포，고온단소골위지가재료，복합구건형성조직공정화골。전마하분비차발제양쌍측하합제일전마아，거제원중근여제이전마아근중근간아조척간격，형성5 mm×5 mm×5 mm골결손구역。장12지실험양수궤등분위조직공정화골조화단순단소골조，분별재좌측하합술구방조직공정화골화단순단소골，소유동물우측균작위공백대조조。 　　결과여결론：고온단소골정백성색，보류료천연송질골적다공상공간。공극솔(66.10±1.32)%，공경범위137.44-538.72μm。간세포접충도단소골24 h후가견세포점부지가상，7 d후분비포외기질，세포여기질분계불청。X 사선가견조직공정화골조화단순단소골조식입재료포매재술구，단소골변연유일권저밀도음영。소목정-이홍염색실험측골소량형성，대조측무명현골생성。제시응용골수간충질간세포복합단소골구건적조직공정화골가교호적수복아조골결손，시수복소범위골결손이상적충자세포화지가재료。
BACKGROUND:Fol owing physicochemical treatment and high-temperature calcinations, heterogeneous biological bone becomes a ceramic-like heterologous bone forming a similar structure to the human bone that is a natural network pore structure, which is conducive to seed cel adhesion and proliferation. OBJECTIVE:To observe the feasibility of constructing tissue-engineered bone through combination of sintered bone and bone marrow mesenchymal stem cel s to repair alveolar defects. METHODS:Sheep bone marrow mesenchymal stem cel s as seed cel s were combined with the high temperature sintered bone as scaffold materials to construct tissue-engineered bone. Under general anesthesia, sheep bilateral mandibular first premolars were removed in batches, the alveolar ridge space between the distal root and mesial root of the second premolar to form a bone defect area of 5 mm×5 mm×5 mm. Twelve experimental sheep were equal y randomized into tissue-engineered bone group and sintered bone group, which were implanted with tissue-engineered bone and sintered bone, respectively, at the left surgical area of the mandible. The right surgical area was considered as blank control group. RESULTS AND CONCLUSION:After high-temperature calcinations, the sintered bone was chalk in color, exhibiting a porous structure as the natural cancel ous bone. The porosity was (66.10±1.32)%, and the pore size was between 137.44μm and 538.72μm. After 24 hours of bone marrow mesenchymal stem cel s inoculated to the sintered bone, a large number of cel s are visible adherent to the scaffold;up to day 7, extracel ular matrix was secreted and there was no clear boundary between the cel s and the matrix. X-ray films showed that the tissue-engineered bone and pure sintered bone implants were embedded in the surgical area, and there was a low-density shadow at the edge of the sintered bone. Hematoxylin-eosin staining showed bone trabecular formation at the experimental side, but no obvious bone formation at the control ed side. Tissue-engineered bone prepared by bone marrow mesenchymal stem cel s and sintered bone can better repair sheep alveolar bone defects, which is an ideal seed cel and scaffold material for smal range bone defects.