中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
35期
6327-6332
,共6页
李瑞琦%张国平%任立中%李亚丽%吕亚军
李瑞琦%張國平%任立中%李亞麗%呂亞軍
리서기%장국평%임립중%리아려%려아군
骨关节植入物%骨与关节综述%骨髓间充质干细胞%股骨头坏死%细胞培养%细胞示踪%细胞分化%省级基金
骨關節植入物%骨與關節綜述%骨髓間充質榦細胞%股骨頭壞死%細胞培養%細胞示蹤%細胞分化%省級基金
골관절식입물%골여관절종술%골수간충질간세포%고골두배사%세포배양%세포시종%세포분화%성급기금
bone and joint implants%review of bone and joint%bone marrow mesenchymal stem cel s%osteonecrosis of femoral head%cel culture%cel tracing%cel differentiation%provincial grants-supported paper
背景:股骨头坏死的治疗方法很多,但目前还没有满意的方法能促进坏死股骨头的修复。近年来,骨髓间充质干细胞移植治疗早期股骨头坏死缺的了一定的效果。<br> 目的:综述骨髓间充质干细胞在股骨头坏死治疗中的应用进展及存在的问题。<br> 方法:应用计算机检索PubMed数据库、万方数据库及CNKI中国知网数据库1999至2012年相关文献,选择骨髓间充质干细胞的分离、培养、分化、细胞标记及体内示踪研究,骨髓间充质干细胞移植治疗股骨头坏死的基础及临床研究,共纳入39篇文献进行综述。<br> 结果与结论:目前骨髓间充质干细胞的分离方法有贴壁筛选法、密度梯度离心法、流式细胞仪分离法和免疫磁珠分选法;常用的细胞标记及示踪法有同位素示踪法、抗原标记法、抗原标记法、荧光染料标记法和 MRI对比增强剂标记法。骨髓间充质干细胞治疗股骨头坏死的方法有髓芯钻孔减压联合骨髓间充质干细胞注射移植治疗股骨头坏死、介入加骨髓间充质干细胞治疗股骨头坏死、基因转染骨髓间充质干细胞移植治疗股骨头坏死、骨髓间充质干细胞的组织工程技术治疗股骨头坏死。虽然近年来对骨髓间充质干细胞移植治疗股骨头缺血坏死的研究取得了很大进展,但仍存在不少问题需要进一步研究。
揹景:股骨頭壞死的治療方法很多,但目前還沒有滿意的方法能促進壞死股骨頭的脩複。近年來,骨髓間充質榦細胞移植治療早期股骨頭壞死缺的瞭一定的效果。<br> 目的:綜述骨髓間充質榦細胞在股骨頭壞死治療中的應用進展及存在的問題。<br> 方法:應用計算機檢索PubMed數據庫、萬方數據庫及CNKI中國知網數據庫1999至2012年相關文獻,選擇骨髓間充質榦細胞的分離、培養、分化、細胞標記及體內示蹤研究,骨髓間充質榦細胞移植治療股骨頭壞死的基礎及臨床研究,共納入39篇文獻進行綜述。<br> 結果與結論:目前骨髓間充質榦細胞的分離方法有貼壁篩選法、密度梯度離心法、流式細胞儀分離法和免疫磁珠分選法;常用的細胞標記及示蹤法有同位素示蹤法、抗原標記法、抗原標記法、熒光染料標記法和 MRI對比增彊劑標記法。骨髓間充質榦細胞治療股骨頭壞死的方法有髓芯鑽孔減壓聯閤骨髓間充質榦細胞註射移植治療股骨頭壞死、介入加骨髓間充質榦細胞治療股骨頭壞死、基因轉染骨髓間充質榦細胞移植治療股骨頭壞死、骨髓間充質榦細胞的組織工程技術治療股骨頭壞死。雖然近年來對骨髓間充質榦細胞移植治療股骨頭缺血壞死的研究取得瞭很大進展,但仍存在不少問題需要進一步研究。
배경:고골두배사적치료방법흔다,단목전환몰유만의적방법능촉진배사고골두적수복。근년래,골수간충질간세포이식치료조기고골두배사결적료일정적효과。<br> 목적:종술골수간충질간세포재고골두배사치료중적응용진전급존재적문제。<br> 방법:응용계산궤검색PubMed수거고、만방수거고급CNKI중국지망수거고1999지2012년상관문헌,선택골수간충질간세포적분리、배양、분화、세포표기급체내시종연구,골수간충질간세포이식치료고골두배사적기출급림상연구,공납입39편문헌진행종술。<br> 결과여결론:목전골수간충질간세포적분리방법유첩벽사선법、밀도제도리심법、류식세포의분리법화면역자주분선법;상용적세포표기급시종법유동위소시종법、항원표기법、항원표기법、형광염료표기법화 MRI대비증강제표기법。골수간충질간세포치료고골두배사적방법유수심찬공감압연합골수간충질간세포주사이식치료고골두배사、개입가골수간충질간세포치료고골두배사、기인전염골수간충질간세포이식치료고골두배사、골수간충질간세포적조직공정기술치료고골두배사。수연근년래대골수간충질간세포이식치료고골두결혈배사적연구취득료흔대진전,단잉존재불소문제수요진일보연구。
BACKGROUND:There are various methods for the treatment of osteonecrosis of femoral head, but there is no satisfactory method to promote the repair of osteonecrosis of femoral head. In recent years, bone marrow <br> mesenchymal stem cel transplantation for the treatment of osteonecrosis of femoral head has achieved certain effect. <br> OBJECTIVE:To review the application progress and problems of bone marrow mesenchymal stem cel transplantation for the treatment of osteonecrosis of femoral head. <br> METHODS:A computer-based online search was performed in PubMed database, Wanfang database and CNKI database for the related articles from 1999 to 2012. The articles on the isolation, culture, differentiation, labeling and in vivo tracing of bone marrow mesenchymal stem cel s were selected, as wel as the basic and clinical <br> researches on bone marrow mesenchymal stem cel transplantation for the treatment of osteonecrosis of femoral head. A total of 39 articles were included for review. <br> RESUTLS AND CONCLUSION:At present, the method for the isolation of bone marrow mesenchymal stem cel s includes adherence screening method, density gradient centrifugation, flow cytometry separation and magnetic activated cel sorting method;the commonly used method for cel labeling and tracing includes isotope tracing method, antigen labeling method, antigen labeling, fluorescent labeling and MRI contrast enhancer labeling <br> method. The method for the treatment of osteonecrosis of femoral head with bone marrow mesenchymal stem cel s includes pith dril ing decompression combined with bone marrow mesenchymal stem cel injection and <br> transplantation, intervention plus bone marrow mesenchymal stem cel transplantation, gene transfection combined with bone marrow mesenchymal stem cel transplantation and tissue engineering technology of bone marrow mesenchymal stem cel s. Although, the research on the bone marrow mesenchymal stem cel transplantation for the treatment of <br> osteonecrosis of femoral head has achieved great progress, there are stil problems needed to be further solved.