CAJ | 학술논문

人类腺病毒可引起呼吸道、胃肠道和眼部的感染,不同类型的腺病毒引起的疾病类型及表现不尽相同.因此,腺病毒的分型具有重要的临床研究价值.在基因学上,编码六邻体蛋白,五邻体蛋白和纤维蛋白的区域是腺病毒基因组中变异最大的区域,通过对其编码区的测序,尤其是六邻体蛋白编码区的测序,是快速可靠的确定腺病毒类型的重要方法之一.此文主要对基因测序在腺病毒分型中的优势、应用现状,在腺病毒突变重组、种系发生学中的鉴定检测等作一综述.
인류선병독가인기호흡도、위장도화안부적감염,불동류형적선병독인기적질병류형급표현불진상동.인차,선병독적분형구유중요적림상연구개치.재기인학상,편마륙린체단백,오린체단백화섬유단백적구역시선병독기인조중변이최대적구역,통과대기편마구적측서,우기시륙린체단백편마구적측서,시쾌속가고적학정선병독류형적중요방법지일.차문주요대기인측서재선병독분형중적우세、응용현상,재선병독돌변중조、충계발생학중적감정검측등작일종술.
Human adenovirus can cause respiratory,gastrointestinal and eye infections.Different adenovirus types can result in different diseases and clinical manifestations.Therefore,adenovirus classification is important for clinical research.Coding region of hexon,penton and fibrin is the largest region of genome variation in adenovirus,and coding region sequencing is one of the rapid and reliable methods for gene typing,especially for hexon protein.The advantage and application of gene sequencing in adenovirus typing,the mutants or recombinants testing and species identification are summarized in this article.