CAJ | 학술논문

　　利用自制的简易生物膜反应器，在3500 lx的光照和14∶10的光暗比下，以Zn2+为模式重金属，利用模拟氮磷废水驯化培养鞘藻（Oedogonium sp.）使其形成藻类生物膜，研究不同Zn2+浓度对鞘藻形成的藻类生物膜生长及生理特性的影响。综合考虑藻类对Zn2+的耐受范围，选定模拟氮磷废水中Zn2+5个质量浓度水平：1.0、3.0、5.0、10.0、20.0 mg·L-1，定时从反应器中取一定量的藻类生物膜，测定以下生理生化指标：叶绿素、超氧化物歧化酶（SOD）、硝酸盐还原酶（NR）、蛋白质及胞外多聚糖，并以藻细胞干质量为基准。研究结果表明：藻类生物膜对Zn2+有一定的耐受性，且质量浓度为5 mg·L-1的Zn2+对藻类生物膜的生长有明显的促进作用，但当质量浓度增大至20 mg·L-1时，藻体大量死亡，藻类生物膜生长明显受到抑制。Zn2+质量浓度为3 mg·L-1的实验组对鞘藻的叶绿素含量有明显的促进作用，而当Zn2+质量浓度为20 mg·L-1时，鞘藻内的叶绿素含量明显减少。不同Zn2+质量浓度对鞘藻的生理生化指标也表现出不同的作用，其中SOD含量随着Zn2+质量浓度的升高明显受到抑制；10 mg·L-1的Zn2+对NR有明显的促进作用；各实验组蛋白质含量在实验初期均有增加，但随之又均有降低；在实验前2 d，20 mg·L-1的Zn2+对胞外多聚糖有明显的促进作用，其含量为对照的1.4倍。以上现象均表明藻类生物膜在重金属的作用下其生长代谢会发生一定的变化，使藻类生物膜能够在一定重金属浓度范围内较好地生长，为藻类生物膜的进一步利用奠定理论基础。
　　이용자제적간역생물막반응기，재3500 lx적광조화14∶10적광암비하，이Zn2+위모식중금속，이용모의담린폐수순화배양초조（Oedogonium sp.）사기형성조류생물막，연구불동Zn2+농도대초조형성적조류생물막생장급생리특성적영향。종합고필조류대Zn2+적내수범위，선정모의담린폐수중Zn2+5개질량농도수평：1.0、3.0、5.0、10.0、20.0 mg·L-1，정시종반응기중취일정량적조류생물막，측정이하생리생화지표：협록소、초양화물기화매（SOD）、초산염환원매（NR）、단백질급포외다취당，병이조세포간질량위기준。연구결과표명：조류생물막대Zn2+유일정적내수성，차질량농도위5 mg·L-1적Zn2+대조류생물막적생장유명현적촉진작용，단당질량농도증대지20 mg·L-1시，조체대량사망，조류생물막생장명현수도억제。Zn2+질량농도위3 mg·L-1적실험조대초조적협록소함량유명현적촉진작용，이당Zn2+질량농도위20 mg·L-1시，초조내적협록소함량명현감소。불동Zn2+질량농도대초조적생리생화지표야표현출불동적작용，기중SOD함량수착Zn2+질량농도적승고명현수도억제；10 mg·L-1적Zn2+대NR유명현적촉진작용；각실험조단백질함량재실험초기균유증가，단수지우균유강저；재실험전2 d，20 mg·L-1적Zn2+대포외다취당유명현적촉진작용，기함량위대조적1.4배。이상현상균표명조류생물막재중금속적작용하기생장대사회발생일정적변화，사조류생물막능구재일정중금속농도범위내교호지생장，위조류생물막적진일보이용전정이론기출。
Using simple biofilm reactor, under 3 500 lx illumination and ratio of light and dark of 14∶10, algal biofilm was domesticated by simulate nitrogen and phosphorus wastewater. The algal species was Oedogonium sp.. The effects of different Zn2+ concentration on algal biofilm formed by Oedogonium sp. was evaluated by the growth and physiological property. Considering algal tolerance range of Zn2+, five concentration levels were selected: 1.0, 3.0, 5.0, 10.0, 20.0 mg Zn2+ per liter simulate nitrogen and phosphorus wastewater. A certain amount of algae biofilm was removed from the reactor regularly. The following physiological biochemical indexes were measured: Chlorophyll, Superoxide Dismutase (SOD), Nitrate Reductase (NR), Protein, Exocellular Polysaccharide. The dry weight was used as the standard. The results showed that: the algal biofilm could endure certain concentration of Zn2+. The Zn2+ concentration of 5 mg·L-1 showed positive promotion effects on algal biofilm growth. When Zn2+ concentration increased to 20 mg·L-1, inhibitory action was obvious. When Zn2+ concentration was 3 mg·L-1, the content of algal chlorophyll was promoted. While the content was decreased under Zn2+ concentration increased to 20 mg·L-1 obviously. The impact of Zn2+on algal biofilm physiological property was different. The content of SOD was obviously restrained with Zn2+ concentration increased. The Zn2+ concentration of 10 mg·L-1 showed positive promotion effects on the content of NR. In the initial stage of test, the content of Protein was increased. However, it was decreased in the last. In the first two days, the Zn2+ concentration of 20 mg·L-1showed positive promotion effects on the content of Exocellular polysaccharide. Its content was 1.4 times than the contrast. The above phenomenon showed that the growth and metabolism of algal biofilm would have some different changes under the impact of heavy metals. These changes could make the algal biofilm grow better under certain concentrations of heavy metals and provide the theoretical basis for the further use of algal biofilm.