CAJ | 학술논문

本文建立了微生物法测定食品中维生素B12（VB12）含量的快速方法，通过低温保藏测试菌液、缩小试验体积，以达到缩短检测周期、简化操作步骤，提高结果准确性与重现性的效果。同时，本文对如何保证试验的有效性和结果的准确性进行了探讨，总结了相关技术要点，为VB12测定微生物法在常规实验室的建立和应用推广提供了参考。
본문건립료미생물법측정식품중유생소B12（VB12）함량적쾌속방법，통과저온보장측시균액、축소시험체적，이체도축단검측주기、간화조작보취，제고결과준학성여중현성적효과。동시，본문대여하보증시험적유효성화결과적준학성진행료탐토，총결료상관기술요점，위VB12측정미생물법재상규실험실적건립화응용추엄제공료삼고。
The article developed a rapid microbiological method for determination of Vitamin B12 in food by storing Lactobacilus leichmannii inoculum at -80 ℃ and minimizing the test volume, which greatly shortened the time and was easy to operate. The article discussed the validity and practicability of the method, summarized the experimental technology to improve the veracity of test results, making routine use of the microbiological assay feasible.