CAJ | 학술논문

　　目的：观察参桃软肝丸含药血清对人肝癌细胞HepG-2细胞凋亡及细胞周期的影响，验证参桃软肝丸可能的作用机理。方法：将9只雄性新西兰家兔分为3组，每组3只，实验分空白对照组、实验组和阳性药物对照组，人肝癌细胞株HepG-2细胞接种于RPMI 1640培养液中，细胞培养为3～5d。MTT法观察细胞抑瘤率，流式细胞仪检测细胞凋亡率和细胞周期。结果：参桃软肝丸对人肝癌细胞株HepG2的生长有抑制作用，且随剂量的增加而增强。其中，高浓度组抑瘤率最高可达43.2%。有较好的诱导凋亡作用，凋亡率可达15.6%，且阻滞细胞增殖于G0/G1期。结论：参桃软肝丸对人肝癌细胞HepG-2有较好的抑瘤和诱导细胞凋亡作用，诱导肿瘤细胞凋亡可能是其抗肿瘤作用机制之一。
　　목적：관찰삼도연간환함약혈청대인간암세포HepG-2세포조망급세포주기적영향，험증삼도연간환가능적작용궤리。방법：장9지웅성신서란가토분위3조，매조3지，실험분공백대조조、실험조화양성약물대조조，인간암세포주HepG-2세포접충우RPMI 1640배양액중，세포배양위3～5d。MTT법관찰세포억류솔，류식세포의검측세포조망솔화세포주기。결과：삼도연간환대인간암세포주HepG2적생장유억제작용，차수제량적증가이증강。기중，고농도조억류솔최고가체43.2%。유교호적유도조망작용，조망솔가체15.6%，차조체세포증식우G0/G1기。결론：삼도연간환대인간암세포HepG-2유교호적억류화유도세포조망작용，유도종류세포조망가능시기항종류작용궤제지일。
Objective:To investigate the Shentao Ruangan pills containing serum on the effect of the HepG-2 cells apoptosis and cycle, and to verify the possible action mechanism of the Shentao Ruangan pill. Methods: 9 New-Zealand male rabbit were randomly divided into three groups, treated group, blank serum group and positive group, 3 for each. The human hepatoma cell line HepG-2 cells were seeded in RPMI 1640 culture medium, for 3~5d. Observing the cell inhibition rate in MTT, and detecting apoptosis rate and cell cycle in flow cytometry. Results:Shentao Ruangan pills have inhibitory effect on the growing of the human hepatoma cell line HepG2, and increased with the dose increasing. Among them, the inhibition rate in the high concentration group was up to 43.2%, had a better induction of apoptosis;apoptosis rate reached 15.6%, and block cell proliferation at G0/G1 phase. Conclusion:Shentao Ruangan pills were effective in inhibition and induce apoptosis of the human hepatoma cell line HepG-2, induce tumor cell apoptosis may be one of the mechanisms of its anti-tumor effect.