CAJ | 학술논문

干细胞%脂肪干细胞%骨髓间充质干细胞%骨髓%脂肪组织%成软骨分化%Ⅱ型胶原%间充质干细胞%省级基金%干细胞图片文章榦細胞%脂肪榦細胞%骨髓間充質榦細胞%骨髓%脂肪組織%成軟骨分化%Ⅱ型膠原%間充質榦細胞%省級基金%榦細胞圖片文章
간세포%지방간세포%골수간충질간세포%골수%지방조직%성연골분화%Ⅱ형효원%간충질간세포%성급기금%간세포도편문장
stem cel s%adipose-derived stem cel s%bone marrow mesenchymal stem cel s%bone marrow%adipose tissue%chondrogenic differentiation%type Ⅱ collagen%mesenchymal stem cel s%provincial grant-supported paper%stem cel photographs-containing paper

　　背景：脂肪干细胞与骨髓间充质干细胞是软骨组织工程中应用较多细胞，二者在生物学特性上有诸多相似之处。目的：比较脂肪来源和骨髓来源的2种间充质干细胞的成软骨分化能力。方法：选取3月龄新西兰大白兔，取腹部脂肪，分离提取脂肪干细胞。取兔双侧股骨，采用贴壁筛选法分离提取骨髓间充质干细胞。绘制第3代脂肪干细胞和骨髓间充质干细胞的生长曲线，比较2种细胞的倍增时间。对第3代脂肪干细胞和骨髓间充质干细胞进行成软骨诱导，分别对诱导14 d的2种细胞行甲苯胺蓝染色和Ⅱ型胶原免疫组化染色。结果与结论：骨髓间充质干细胞原代细胞呈聚集样生长，而脂肪干细胞原代细胞呈单个、散在生长。脂肪干细胞增殖速度要快于骨髓间充质干细胞，倍增时间短于骨髓间充质干细胞h。2种细胞成软骨诱导14 d后，均表达糖胺聚糖和Ⅱ型胶原，且骨髓间充质干细胞成软骨诱导后表达Ⅱ型胶原水平高于脂肪干细胞。说明脂肪干细胞与骨髓间充质干细胞体外增殖皆迅速且稳定，但是脂肪干细胞的生长增殖速度更快。单层培养时，特定条件下，脂肪干细胞与骨髓间充质干细胞均能向软骨细胞转化，但是骨髓间充质干细胞比脂肪干细胞具有更高的潜能。
　　배경：지방간세포여골수간충질간세포시연골조직공정중응용교다세포，이자재생물학특성상유제다상사지처。목적：비교지방래원화골수래원적2충간충질간세포적성연골분화능력。방법：선취3월령신서란대백토，취복부지방，분리제취지방간세포。취토쌍측고골，채용첩벽사선법분리제취골수간충질간세포。회제제3대지방간세포화골수간충질간세포적생장곡선，비교2충세포적배증시간。대제3대지방간세포화골수간충질간세포진행성연골유도，분별대유도14 d적2충세포행갑분알람염색화Ⅱ형효원면역조화염색。결과여결론：골수간충질간세포원대세포정취집양생장，이지방간세포원대세포정단개、산재생장。지방간세포증식속도요쾌우골수간충질간세포，배증시간단우골수간충질간세포h。2충세포성연골유도14 d후，균표체당알취당화Ⅱ형효원，차골수간충질간세포성연골유도후표체Ⅱ형효원수평고우지방간세포。설명지방간세포여골수간충질간세포체외증식개신속차은정，단시지방간세포적생장증식속도경쾌。단층배양시，특정조건하，지방간세포여골수간충질간세포균능향연골세포전화，단시골수간충질간세포비지방간세포구유경고적잠능。
BACKGROUND:Adipose-derived stem cel s and bone marrow mesenchymal stem cel s are used widely in cartilage tissue engineering, and there are many similarities in biological characteristics between two kinds of cel s. OBJECTIVE:To compare the chondrogenic potential of bone marrow mesenchymal stem cel s and adipose-derived stem cel s in vitro. METHODS:Adipose-derived stem cel s were isolated from the 3-month-old New Zealand white rabbits’ abdomen. Bilateral femurs of rabbits were obtained, and then the bone marrow mesenchymal stem cel s were separated with the adherence screening method. The growth curve of the passage 3 adipose-derived stem cel s and bone marrow mesenchymal stem cel s were drawn, and the doubling time of two kinds of cel s was compared. Then the passage 3 adipose-derived stem cel s and bone marrow mesenchymal stem cel s were treated with chondrogenic induction. After induced for 14 days, the adipose-derived stem cel s and bone marrow mesenchymal stem cel s were treated with toluidine blue staining and type Ⅱ immunohistochemistry staining respectively. RESULTS AND CONCLUSION:Primary bone marrow mesenchymal stem cel s showed aggregative growth, while the primary adipose-derived stem cel s were in single and scattered growth. The proliferation speed of adipose-derived stem cel s was faster than that of bone marrow mesenchymal stem cel s, while the doubling time of adipose-derived stem cel s was shorter than that of the bone marrow mesenchymal stem cel s. After chondrogenic induction for 14 days, both adipose-derived stem cel s and bone marrow mesenchymal stem cel s could express glycosaminoglycans and type Ⅱcol agen, and the expression level of type Ⅱ col agen in bone marrow mesenchymal stem cel s after chondrogenic induction was higher than that in the adipose-derived stem cel s. The in vitro proliferation of adipose-derived stem cel s and bone marrow mesenchymal stem cel s was rapid and stable, but the proliferative ability of adipose-derived stem cel s was faster than that of bone marrow mesenchymal stem cel s. When cultured in single layer, both adipose-derived stem cel s and bone marrow mesenchymal stem cel s could transform into chondrocytes under certain conditions, but bone marrow mesenchymal stem cel s seemed to be more potential than adipose-derived stem cel s.