CAJ | 학술논문

干细胞%脐带脐血干细胞%人脐带间充质干细胞%氧化应激%活性氧%衰老%谷胱甘肽%丙二醛%超氧化物歧化酶%应激水平%干细胞图片文章榦細胞%臍帶臍血榦細胞%人臍帶間充質榦細胞%氧化應激%活性氧%衰老%穀胱甘肽%丙二醛%超氧化物歧化酶%應激水平%榦細胞圖片文章
간세포%제대제혈간세포%인제대간충질간세포%양화응격%활성양%쇠로%곡광감태%병이철%초양화물기화매%응격수평%간세포도편문장
stem cel s%umbilical cord/umbilical blood stem cel s%human umbilical cord-derived mesenchymal stem cel s%oxidative stress%active oxygen%senescence%glutathione%malondialdehyde%superoxide dismutase%stress level%stem cel photographs-containing paper

　　背景：目前临床移植常用的保存液可使人脐带来源间充质干细胞活性下降，影响移植效果，但关于其活性下降原因的报道目前还很少。目的：探索氧化应激是否为人脐带来源间充质干细胞临床移植保存过程中活性下降的因素；并在保存液中添加自由基清除剂是否可提高保存效果。方法：室温下用生理盐水保存人脐带来源间充质干细胞，0，2，4，6 h后分别检测细胞内活性氧水平、检测丙二醛含量以及抗氧化酶活性以判定保存后细胞内氧化应激水平；保存液中添加自由基清除剂N-乙酰半胱氨酸后检测细胞贴壁率变化。结果与结论：经生理盐水保存后人脐带来源间充质干细胞内活性氧水平升高，细胞裂解液丙二醛含量呈时间依赖性增加，抗氧化酶超氧化物歧化酶、过氧化氢酶以及谷胱甘肽过氧化物酶活性均降低。添加N-乙酰半胱氨酸的移植保存液组比不添加组保存人脐带来源间充质干细胞后细胞活性氧水平降低、贴壁率升高。说明在临床常用移植保存液中人脐带来源间充质干细胞内活性氧升高是其活性下降的一个因素，在保存液中添加自由基清除剂N-乙酰半胱氨酸后能够一定程度的改善保存效果。
　　배경：목전림상이식상용적보존액가사인제대래원간충질간세포활성하강，영향이식효과，단관우기활성하강원인적보도목전환흔소。목적：탐색양화응격시부위인제대래원간충질간세포림상이식보존과정중활성하강적인소；병재보존액중첨가자유기청제제시부가제고보존효과。방법：실온하용생리염수보존인제대래원간충질간세포，0，2，4，6 h후분별검측세포내활성양수평、검측병이철함량이급항양화매활성이판정보존후세포내양화응격수평；보존액중첨가자유기청제제N-을선반광안산후검측세포첩벽솔변화。결과여결론：경생리염수보존후인제대래원간충질간세포내활성양수평승고，세포렬해액병이철함량정시간의뢰성증가，항양화매초양화물기화매、과양화경매이급곡광감태과양화물매활성균강저。첨가N-을선반광안산적이식보존액조비불첨가조보존인제대래원간충질간세포후세포활성양수평강저、첩벽솔승고。설명재림상상용이식보존액중인제대래원간충질간세포내활성양승고시기활성하강적일개인소，재보존액중첨가자유기청제제N-을선반광안산후능구일정정도적개선보존효과。
BACKGROUND:The viability of human umbilical cord-derived mesenchymal stem cel s is often declined with the commonly used transplantation storage solution in clinics, which may influence the therapeutic effects of cel ular transplantation. However, reasons for this are stil unknown. OBJECTIVE:To investigate the role of oxidative stress in the reduction of human umbilical cord-derived mesenchymal stem cel s viability in the storage process during clinical transplantation and to observe the effects of radical scavenger on the results. METHODS:Human umbilical cord-derived mesenchymal stem cel s were harvested and cultured in normal saline for 0, 2, 4 and 6 hours at room temperature. Intracel ular reactive oxygen levels were detected at those time points. Antioxidant enzyme activities and levels of malondialdehyde were measured to determine the intracel ular oxidative stress levels after storage. Cel adhesion rate changes were retested after adding N-acetyl cysteine to the storage solution. RESULTS AND CONCLUSION:The reactive oxygen levels in human umbilical cord-derived mesenchymal stem cel s were increased significantly after normal saline storage and levels of malondialdehyde were increased in a time-dependent manner. Activities of superoxide dismutase, catalase and glutathione peroxidase were al reduced. Addition of N-acetyl cysteine into the storage medium decreased the reactive oxygen levels and improved the human umbilical cord-derived mesenchymal stem cel s viabilities. Experimental findings indicate that, increased reactive oxygen species in human umbilical cord-derived mesenchymal stem cel s is one of the reasons for reduced cel viability. Adding the radical scavenger N-acetyl cysteine can improve the storage effects of human umbilical cord-derived mesenchymal stem cel s.