CAJ | 학술논문

　　背景：目前骨髓间充质干细胞向神经细胞分化的方法较多，采用不同诱导方法对骨髓充质干细胞分化成神经细胞的比例是不同的。目的：比较化学诱导法和共培养法诱导大鼠骨髓间充质干细胞分化为神经细胞的差异。方法：大鼠全骨髓血细胞分离纯化法培养骨髓间充质干细胞，分为化学诱导组和共培养组，分别采用加入化学诱导剂β-巯基乙醇和Transwel 小室共培养方法诱导骨髓间充质干细胞向神经细胞分化。结果与结论：诱导培养1周后从化学诱导和共培养组的骨髓间充质干细胞出现突起，且呈辐射生长，2周后均可见神经元特异性烯醇化酶阳性细胞。共培养组中第四五天可见星级神经细胞状结构，并形成更多的突起，神经元特异性烯醇化酶染色阳性率为(70.82±2.46)%。而在第六七天化学诱导组中神经细胞形态样细胞形成，并有连接，神经元特异性烯醇化酶染色阳性率为(52.37±1.83)%。提示细胞微环境在骨髓间充质干细胞分化成神经细胞发挥主导作用，且化学诱导法诱导效率低于共培养法。
　　배경：목전골수간충질간세포향신경세포분화적방법교다，채용불동유도방법대골수충질간세포분화성신경세포적비례시불동적。목적：비교화학유도법화공배양법유도대서골수간충질간세포분화위신경세포적차이。방법：대서전골수혈세포분리순화법배양골수간충질간세포，분위화학유도조화공배양조，분별채용가입화학유도제β-구기을순화Transwel 소실공배양방법유도골수간충질간세포향신경세포분화。결과여결론：유도배양1주후종화학유도화공배양조적골수간충질간세포출현돌기，차정복사생장，2주후균가견신경원특이성희순화매양성세포。공배양조중제사오천가견성급신경세포상결구，병형성경다적돌기，신경원특이성희순화매염색양성솔위(70.82±2.46)%。이재제륙칠천화학유도조중신경세포형태양세포형성，병유련접，신경원특이성희순화매염색양성솔위(52.37±1.83)%。제시세포미배경재골수간충질간세포분화성신경세포발휘주도작용，차화학유도법유도효솔저우공배양법。
BACKGROUND:Currently, bone marrow mesenchymal stem cel s can differentiate into nerve cel s via many approaches. Different methods for inducing bone marrow mesenchymal stem cel s differentiating into nerve cel s have different ratios. OBJECTIVE:To investigate the difference between chemical method and co-culture method to induce the differentiation of rat bone marrow mesenchymal stem cel s into nerve cel s. METHODS:Rat bone marrow mesenchymal stem cel s were isolated and purified using whole bone marrow culture method, and then randomly divided into two groups:chemical group,β-mercaptoethanol was added;co-culture group, co-cultured in a Transwel chamber. RESULTS AND CONCLUSION:Visible protrusions from induced cel s showed radiation growth at 1 week of induced culture, and neuron-specific enolase staining was positive at 2 weeks of culture. Star-like structure of nerve cel s was visible in the co-culture group within 4-5 days of culture, and then more protrusions formed. Meanwhile, the positive rate of neuron-specific enolase was (70.82±2.46)%. After 6-7 days of culture, neuron-like cel s formed and were interconnected in the chemical group;while, the positive rate of neuron-specific enolase was (52.37±1.83)%. These findings suggest that cel microenvironment plays a leading role in the differentiation of bone marrow mesenchymal stem cel s into nerve cel s, and chemical induction method is inferior to the co-culture method.