中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
32期
5834-5839
,共6页
罗宏武%寻权%黄湘俊%黄飞舟
囉宏武%尋權%黃湘俊%黃飛舟
라굉무%심권%황상준%황비주
干细胞%干细胞移植%人羊膜上皮细胞%脾内移植%急性肝损伤%肝功能%人血白蛋白%谷丙转氨酶%谷草转氨酶%定量分析%肝脏%脾脏%干细胞图片文章
榦細胞%榦細胞移植%人羊膜上皮細胞%脾內移植%急性肝損傷%肝功能%人血白蛋白%穀丙轉氨酶%穀草轉氨酶%定量分析%肝髒%脾髒%榦細胞圖片文章
간세포%간세포이식%인양막상피세포%비내이식%급성간손상%간공능%인혈백단백%곡병전안매%곡초전안매%정량분석%간장%비장%간세포도편문장
背景:目前已有较多关于人羊膜上皮细胞移植入动物体内的存活、迁徙及相关特性的初步研究,但其对移植效果的定量分析尚未见报道。目的:对脾内移植传代的人羊膜上皮细胞小鼠血清肝生化功能及人血白蛋白的定量分析。方法:40只裸小鼠随机分为4组,每组各10只。肝叶切除+细胞移植2周组、肝叶切除+细胞移植4周组、肝叶切除+盐水组,行半肝叶切除,肝叶切除+细胞移植组自脾下极移植密度为5×106传代的人羊膜上皮细胞约0.2 mL,分别于移植后2周和4周采血;肝叶切除+盐水组自脾下极注射生理盐水0.2 mL;单纯细胞移植组:不行肝叶切除,自脾下极移植密度为5×106传代的人羊膜上皮细胞约0.2 mL。检测其各组肝脾组织学、形态学的改变及各组血清谷丙转氨酶、谷草转氨酶、人血白蛋白的变化和人血白蛋白表达定量分析。结果与结论:人羊膜上皮细胞移植急性肝损伤小鼠4周后肝脾形态未见明显改变,组织学可检测到特异性细胞,血清谷丙转氨酶、谷草转氨酶、人血白蛋白有明显改善,血清中能检测到人血白蛋白且移植后4周较移植后2周有明显升高。因此,人羊膜上皮细胞移植入肝受损小鼠体内能存活超过4周且仍表达肝细胞样细胞的部分特性及功能,改善小鼠的肝功能,治疗小鼠急性肝损伤。
揹景:目前已有較多關于人羊膜上皮細胞移植入動物體內的存活、遷徙及相關特性的初步研究,但其對移植效果的定量分析尚未見報道。目的:對脾內移植傳代的人羊膜上皮細胞小鼠血清肝生化功能及人血白蛋白的定量分析。方法:40隻裸小鼠隨機分為4組,每組各10隻。肝葉切除+細胞移植2週組、肝葉切除+細胞移植4週組、肝葉切除+鹽水組,行半肝葉切除,肝葉切除+細胞移植組自脾下極移植密度為5×106傳代的人羊膜上皮細胞約0.2 mL,分彆于移植後2週和4週採血;肝葉切除+鹽水組自脾下極註射生理鹽水0.2 mL;單純細胞移植組:不行肝葉切除,自脾下極移植密度為5×106傳代的人羊膜上皮細胞約0.2 mL。檢測其各組肝脾組織學、形態學的改變及各組血清穀丙轉氨酶、穀草轉氨酶、人血白蛋白的變化和人血白蛋白錶達定量分析。結果與結論:人羊膜上皮細胞移植急性肝損傷小鼠4週後肝脾形態未見明顯改變,組織學可檢測到特異性細胞,血清穀丙轉氨酶、穀草轉氨酶、人血白蛋白有明顯改善,血清中能檢測到人血白蛋白且移植後4週較移植後2週有明顯升高。因此,人羊膜上皮細胞移植入肝受損小鼠體內能存活超過4週且仍錶達肝細胞樣細胞的部分特性及功能,改善小鼠的肝功能,治療小鼠急性肝損傷。
배경:목전이유교다관우인양막상피세포이식입동물체내적존활、천사급상관특성적초보연구,단기대이식효과적정량분석상미견보도。목적:대비내이식전대적인양막상피세포소서혈청간생화공능급인혈백단백적정량분석。방법:40지라소서수궤분위4조,매조각10지。간협절제+세포이식2주조、간협절제+세포이식4주조、간협절제+염수조,행반간협절제,간협절제+세포이식조자비하겁이식밀도위5×106전대적인양막상피세포약0.2 mL,분별우이식후2주화4주채혈;간협절제+염수조자비하겁주사생리염수0.2 mL;단순세포이식조:불행간협절제,자비하겁이식밀도위5×106전대적인양막상피세포약0.2 mL。검측기각조간비조직학、형태학적개변급각조혈청곡병전안매、곡초전안매、인혈백단백적변화화인혈백단백표체정량분석。결과여결론:인양막상피세포이식급성간손상소서4주후간비형태미견명현개변,조직학가검측도특이성세포,혈청곡병전안매、곡초전안매、인혈백단백유명현개선,혈청중능검측도인혈백단백차이식후4주교이식후2주유명현승고。인차,인양막상피세포이식입간수손소서체내능존활초과4주차잉표체간세포양세포적부분특성급공능,개선소서적간공능,치료소서급성간손상。
BACKGROUND:There are many preliminary studies on the survival, metaptosis, and correlation characteristics of human amniotic epithelial cel s after transplanted into the animals, but there are no reports on the quantitative analysis of the transplantation effect. OBJECTIVE:To make quantitative analysis on serum biochemical function of liver and the expression of human albumin in mice received passaged human amniotic epithelial cel s transplantation in spleen. METHODS:Forty nude mice were randomly divided into four groups (n=10 in each group):hepatectomy+cel transplantation 2 weeks group, hepatectomy+cel transplantation 4 weeks group, hepatectomy+normal saline group (treated with partial hepatectomy) and hepatectomy+cel transplantation group (transplanted with 0.2 mL passaged human amniotic epithelial cel s with 5×106 under spleen, and the blood were col ected at 2 and 4 weeks after transplantation). The mice in the hepatectomy+normal saline group were treated with splenic injection of 0.2 mL normal saline;the cel transplantation group did not receive hepatectomy, and transplanted with 0.2 mL passaged human amniotic epithelial cel s with 5×106 under spleen. The histological and morphological changes of the liver and spleen in each group as wel as the expressions of serum alanine aminotransferase, aspartate aminotransferase and human serum albumin in each group were detected, and the quantitative analysis of human serum albumin expression was performed. RESULTS AND CONCLUSION: There was no obvious morphological change after human amniotic epithelial cel s transplanted into the acute liver injury mice for 4 weeks, but specific cel s could be detected by histological method. The serum levels of alanine aminotransferase, aspartate aminotransferase and human serum albumin were improved obviously, and the human albumin could be detected in serum, the level of human albumin at 4 weeks after transplantation was significantly increased than 2 weeks after transplantation. Human amniotic epithelial cel s can survive for more than 4 weeks after transplanted into the liver injury mice, and can stil express partial characteristics and functions of hepatocyte-like cel s, improve the liver function, thus treating acute liver injury.