CAJ | 학술논문

目的比较3种不同食物诱导的大鼠脂肪肝模型中脂肪变类型及代谢表型之间的差异.方法将雄性LEWs大鼠随机分为4组,分别给予普通饲料(NC组)、胆碱-蛋氨酸缺乏饲料(MCD组)、低胆碱-蛋氨酸加高脂饲料(MCD+ HF组)和低胆碱-蛋氨酸加高糖饲料(FLD组)饲养l、2、4、6周及3个月.评估不同组各时间点大鼠肝脏脂肪变的程度,观察脂肪变病理类型、肝脏酶学改变及脂质过氧化和抗氧化水平变化.结果 MCD组大鼠体质量减轻约30％并有超过66％的肝脏细胞脂肪变性,肝脏酶学以谷丙转氨酶(ALT)升高为主(与NC组比较,P＜O.05),脂质过氧化产物(LP0)较NC组升高160倍(P＜0.05),谷胱甘肽(GSH)含量第1周与NC组比较,差异无统计学意义(P＞0.05),随着饲养时间的延长逐渐降低至MCD+ HF组及FLD组水平；MCD+ HF组大鼠体质量曲线与NC组比较,差异无统计学意义(P＞0.05),并有超过66％的肝脏细胞脂肪变性,以谷草转氨酶(AST)升高为主,LPO升高约4倍(与NC组比较,P＜0.05),GSH含量自第1周起较NC组降低(与NC组比较,P＜0.05)；FLD组大鼠体质量曲线与NC组比较,差异无统计学意义(JP＞0.05),但仅表现轻度脂肪变,其肝脏酶学结果与NC组相似,但其LPO及GSH变化与MCD+ HF组相似.结论不同的食物诱导的脂肪肝形成机制不同,这可能导致了脂肪肝研究中不同的实验结果.
목적 비교3충불동식물유도적대서지방간모형중지방변류형급대사표형지간적차이.방법 장웅성LEWs대서수궤분위4조,분별급여보통사료(NC조)、담감-단안산결핍사료(MCD조)、저담감-단안산가고지사료(MCD+ HF조)화저담감-단안산가고당사료(FLD조)사양l、2、4、6주급3개월.평고불동조각시간점대서간장지방변적정도,관찰지방변병리류형、간장매학개변급지질과양화화항양화수평변화.결과 MCD조대서체질량감경약30％병유초과66％적간장세포지방변성,간장매학이곡병전안매(ALT)승고위주(여NC조비교,P＜O.05),지질과양화산물(LP0)교NC조승고160배(P＜0.05),곡광감태(GSH)함량제1주여NC조비교,차이무통계학의의(P＞0.05),수착사양시간적연장축점강저지MCD+ HF조급FLD조수평；MCD+ HF조대서체질량곡선여NC조비교,차이무통계학의의(P＞0.05),병유초과66％적간장세포지방변성,이곡초전안매(AST)승고위주,LPO승고약4배(여NC조비교,P＜0.05),GSH함량자제1주기교NC조강저(여NC조비교,P＜0.05)；FLD조대서체질량곡선여NC조비교,차이무통계학의의(JP＞0.05),단부표현경도지방변,기간장매학결과여NC조상사,단기LPO급GSH변화여MCD+ HF조상사.결론 불동적식물유도적지방간형성궤제불동,저가능도치료지방간연구중불동적실험결과.
Objective To evaluate the effect of nutrition diets on rats and compare the steatosis and metabolic phenotype in liver.Methods Male LEWs rats were fed with low methionine/choline + high starch diet (FLD),low methionine/choline + high fat diet (MCD + HF),methionine-choline deficient diet (MCD) and standard diet (NC) for 1,2,4,6 weeks and 3 months.Hepatic steatosis,liver enzymes,histological features,lipid peroxidation products (LPO) and glutathione (GSH) were measured.Results MCD diet caused severe body weight loss (30％) and severe steatosis.A substantial release of ALT was observed.Induction of LPO increased up to 160 fold (P ＜ 0.05 versus NC group) and was accompanied by an increasing but mild depletion of GSH.MCD + HF diet induced severe steatosis and the body weight of rats was similar to the level of NC group.A substantial release of AST was observed.The oxidative stress was present by a 4-fold elevation of LPO and depletion of GSH.FLD diet did not induced severe steatosis and body weight loss,but induced oxidative stress which was similar to MCD + HF group.Conclusion Different dietary regimens caused steatosis of comparable severity,but differed substantially in respect to the metabolic phenotype.These findings may contribute to the controversial discussion in fatty liver studies.