CAJ | 학술논문

目的探讨梗阻性黄疸患者胆汁中的微生物群落结构.方法应用末端标记限制性片段长度多态性(T-RFLP)和克隆文库分析,对昆明医科大学第二附属医院肝胆胰外科三病区2010年10月至2013年10月期间诊断为梗阻性黄疸患者的胆汁进行细菌培养,培养结果为阴性的患者胆汁再进行微生物群落结构进行分析.结果共117例患者的胆汁纳入研究,胆汁中细菌16S核糖体DNA (rDNA)阳性率为42.7％ (50/117).不同梗阻原因胆汁中细菌16S rDNA阳性率(97.3％比17.5％)差异有统计学意义(P＜0.05),而梗阻位置相同的细菌16S rDNA阳性率(43.3％比38.1％)差异无统计学意义(P＞0.05).结论采用T-RFLP方法分析16S rDNA克隆片段能够有效评估梗阻性黄疸患者胆汁中的细菌群落存在和多样性.
목적 탐토경조성황달환자담즙중적미생물군락결구.방법 응용말단표기한제성편단장도다태성(T-RFLP)화극륭문고분석,대곤명의과대학제이부속의원간담이외과삼병구2010년10월지2013년10월기간진단위경조성황달환자적담즙진행세균배양,배양결과위음성적환자담즙재진행미생물군락결구진행분석.결과 공117례환자적담즙납입연구,담즙중세균16S핵당체DNA (rDNA)양성솔위42.7％ (50/117).불동경조원인담즙중세균16S rDNA양성솔(97.3％비17.5％)차이유통계학의의(P＜0.05),이경조위치상동적세균16S rDNA양성솔(43.3％비38.1％)차이무통계학의의(P＞0.05).결론 채용T-RFLP방법분석16S rDNA극륭편단능구유효평고경조성황달환자담즙중적세균군락존재화다양성.
Objective To determine the microbial community structure in bile from obstructive jaundice.Methods Structure of bile microbial community from obstructive jaundice patents was investigated by terminal restriction fragment length polymorphism (T-RFLP)and clone libraries approaches.16S ribosomal DNA (16S rDNA) was retrieved from the patients with obstructive jaundice and negative bile culture from October 2010 to October 2013 in our ward.Results 117 cases of patients included in the study of bile.According to percentage of 16S rDNA stone from 50 patients with negative bile culture.The positive rate of bacterial 16S rDNA was 42.7％ (50/117).The positive rate of bacterial 16S rDNA have different reasons for biliary obstruction in a significant difference (97.3％ vs.17.5％),but there is no statistical difference between the position of obstruction (43.3％ vs.38.1％).Conclusion The result illuminated that T-RFLP analysis of cloned 16S rDNA fragments is a powerful tool for estimating the community an diversity of bacterial in bile sample from obstructive jaundice.