CAJ | 학술논문

目的：探讨整合素在CCL18促进乳腺癌SK-3rd细胞浸润和迁移过程中的作用，以阐明CCL18促进乳腺癌细胞浸润和迁移的分子机制。方法用流式细胞检测技术检测CCL18作用下乳腺癌SK-3rd细胞表面整合素的聚集；采用Western blot检测黏着斑激酶（FAK）的激活；用浸润迁移实验检测乳腺癌SK-3rd细胞的浸润迁移。用siRNA转染检测沉默SK-3rd细胞整合素β1的表达。结果 CCL18促进乳腺癌SK-3rd细胞表面整合素的聚集；从而促进整合素介导的FAK磷酸化激活。在CCL18作用下，乳腺癌SK-3rd细胞浸润和迁移的细胞数增多10倍（P＜0．01）；用 siRNA转染检测沉默 SK-3rd细胞整合素β1的表达，可以使CCL18作用下的SK-3rd细胞浸润和迁移数量明显减少。结论 CCL18通过整合素聚集可促进乳腺癌细胞浸润和迁移。
목적：탐토정합소재CCL18촉진유선암SK-3rd세포침윤화천이과정중적작용，이천명CCL18촉진유선암세포침윤화천이적분자궤제。방법용류식세포검측기술검측CCL18작용하유선암SK-3rd세포표면정합소적취집；채용Western blot검측점착반격매（FAK）적격활；용침윤천이실험검측유선암SK-3rd세포적침윤천이。용siRNA전염검측침묵SK-3rd세포정합소β1적표체。결과 CCL18촉진유선암SK-3rd세포표면정합소적취집；종이촉진정합소개도적FAK린산화격활。재CCL18작용하，유선암SK-3rd세포침윤화천이적세포수증다10배（P＜0．01）；용 siRNA전염검측침묵 SK-3rd세포정합소β1적표체，가이사CCL18작용하적SK-3rd세포침윤화천이수량명현감소。결론 CCL18통과정합소취집가촉진유선암세포침윤화천이。
Objective To explore the role of integrin in CCL18 for promoting breast cancer SK-3 rd cells invasion and migration process to illuminate the molecular mechanism of CCL 18 for promoting breast cancer SK-3 rd cells invasion and migration process . Methods The flow cytometry was adopted to detect CCL18-induced integrin aggregation ;Western blot was used to detect the focal adhesion kinase(FAK ) activation ,the infiltrating migrationin experiment was adopted to determine the invasion and migration of SK-3rd cells and the siRNAs transfection was used to detect the expression of silence integrin β1 .Results CCL18 promoted the in-tegrinβ1 aggregation in breast cancer SK-3rd cell surface and further promoted the integrin-mediated phosphorylated activation of FAK .Under the reaction of CCL18 ,the cells number of SK-3rd cellular invasion and migration was increased by ten times (P<0 .01) ,which was obviously decreased by siRNA silenced integrin β1 .Conclusion CCL18 promotes breast cancer invasion and mi-gration via integrin aggregation .