CAJ | 학술논문

　　目的：分析Hp阳性和Hp阴性的胃癌患者外周血单核细胞来源树突状细胞（MODCs）功能的差异性及其临床意义。方法：用尿素14C呼气试验对解放军第一七四医院2011年1月至2012年10月收治的84例胃癌患者进行Hp感染状态鉴定，分别采集Hp阳性和阴性胃癌患者外周血，分离外周血单个核细胞，采用经典方法（rhGM-CSF、rhIL-4联合rhTNF-α）诱导产生DCs，采用流式细胞仪检测DCs表型，采用LDH释放法检测DCs致敏T细胞对胃癌细胞的毒性杀伤作用，采用ELISA方法检测细胞因子IL-12、IFN-γ的分泌水平。结果：两组MoDCs成熟过程形态变化无差异，Hp阳性组MoDCs表面标记分子CD1a、CD80、CD83、CD86和HLA-DR平均表达百分率均高于Hp阴性组，其中CD80、CD83、CD86的表达水平差异有统计学意义，CD1a、HLA-DR差异无统计学意义。Hp阳性组DCs致敏T淋巴细胞对胃癌细胞杀伤率和IL-12、IFN-γ的分泌水平均高于Hp阴性组（P<0.05）。结论：Hp感染状态不影响胃癌患者外周血MoDCs成熟过程形态变化，Hp感染的胃癌患者MoDCs成熟和活化水平更高。
　　목적：분석Hp양성화Hp음성적위암환자외주혈단핵세포래원수돌상세포（MODCs）공능적차이성급기림상의의。방법：용뇨소14C호기시험대해방군제일칠사의원2011년1월지2012년10월수치적84례위암환자진행Hp감염상태감정，분별채집Hp양성화음성위암환자외주혈，분리외주혈단개핵세포，채용경전방법（rhGM-CSF、rhIL-4연합rhTNF-α）유도산생DCs，채용류식세포의검측DCs표형，채용LDH석방법검측DCs치민T세포대위암세포적독성살상작용，채용ELISA방법검측세포인자IL-12、IFN-γ적분비수평。결과：량조MoDCs성숙과정형태변화무차이，Hp양성조MoDCs표면표기분자CD1a、CD80、CD83、CD86화HLA-DR평균표체백분솔균고우Hp음성조，기중CD80、CD83、CD86적표체수평차이유통계학의의，CD1a、HLA-DR차이무통계학의의。Hp양성조DCs치민T림파세포대위암세포살상솔화IL-12、IFN-γ적분비수평균고우Hp음성조（P<0.05）。결론：Hp감염상태불영향위암환자외주혈MoDCs성숙과정형태변화，Hp감염적위암환자MoDCs성숙화활화수평경고。
Objective: This study aimed to compare and analyze the functional differences between peripheral blood mono-cyte-derived dendritic cells (DCs) of Helicobacter pylori-positive and H. pylori-negative patients with gastric cancer. Methods:H. py-lori infection was detected in 84 patients with gastric cancer in our hospital from January 2011 to October 2012 by the 14C-urea breath test. DCs were generated from monocytes isolated by an adherent method from the two groups of patients and cultured in the presence of rhIL-4, rhGM-CSF, and rhTNF-α. Furthermore, the expression of surface marker molecules was determined by fluorescence-activat-ed cell sorting analysis. The cytotoxicity of DCs pulsed T cells against gastric carcinoma cell was assessed by the lactate dehydroge-nase-releasing assay. The secretion of IL-12 and IFN-γin the supernatant was determined by enzyme-linked immunosorbent assay. Re-sults:No difference was observed in the morphological change of the maturation process. The mean expression of CD1a, CD80, CD83, CD86, and HLA-DR molecules in DCs of H. pylori-infected patients was higher than that in DCs of H. pylori-negative group, and the differences were statistically significant except for CD1a and HLA-DR. The cytotoxicity activities, IL-12 release, and IFN-γrelease in the H. pylori-positive group were significantly higher than those in the H. pylori-negative group (P<0.05). Conclusion:H. pylori infec-tion has no effect on the morphological change of the maturation process of monocyte-derived DCs. These data clearly demonstrate that monocyte-derived DCs of H. pylori-infected patients with gastric cancer can induce stronger maturation and activation than those of H. pylori-negative patients.