CAJ | 학술논문

　　目的：本研究旨在采用99mTc-HYNIC-annexinⅤ活体检测放疗后肿瘤的凋亡情况，并初步探讨凋亡与照射剂量及肿瘤敏感性的关系。方法：将EL4淋巴瘤和S180肉瘤细胞株分别接种于实验小鼠右腋下10 d，随机分成显像组和观察组。显像组不同剂量照射后尾静脉注射99mTc-HYNIC-annexinⅤ，2 h后SPECT显像，取组织称重后分别测量放射性计数，计算每克组织百分注射剂量率（%ID/g）及T/B、T/M放射性比值，应用TUNEL法检测肿瘤凋亡细胞数。观察组照射后观察2周。结果：EL4淋巴瘤随照射剂量增加显影逐渐清晰，凋亡细胞数增多，且与%ID/g呈正相关（r=0.892，P<0.001）；S180肉瘤不明显。相同剂量（0 Gy或8 Gy）照射，EL4淋巴瘤放射性分布及凋亡细胞数明显高于S180肉瘤。8 Gy照射后，S180肉瘤仅缩小0.1 cm，EL4淋巴瘤完全消退。结论：99mTc-HYNIC-annexinⅤ可早期活体检测放疗诱导的肿瘤凋亡。放射诱导的凋亡与疗效呈正相关，检测凋亡有助于判断其对放疗的敏感性，可以作为预后的指标。
　　목적：본연구지재채용99mTc-HYNIC-annexinⅤ활체검측방료후종류적조망정황，병초보탐토조망여조사제량급종류민감성적관계。방법：장EL4림파류화S180육류세포주분별접충우실험소서우액하10 d，수궤분성현상조화관찰조。현상조불동제량조사후미정맥주사99mTc-HYNIC-annexinⅤ，2 h후SPECT현상，취조직칭중후분별측량방사성계수，계산매극조직백분주사제량솔（%ID/g）급T/B、T/M방사성비치，응용TUNEL법검측종류조망세포수。관찰조조사후관찰2주。결과：EL4림파류수조사제량증가현영축점청석，조망세포수증다，차여%ID/g정정상관（r=0.892，P<0.001）；S180육류불명현。상동제량（0 Gy혹8 Gy）조사，EL4림파류방사성분포급조망세포수명현고우S180육류。8 Gy조사후，S180육류부축소0.1 cm，EL4림파류완전소퇴。결론：99mTc-HYNIC-annexinⅤ가조기활체검측방료유도적종류조망。방사유도적조망여료효정정상관，검측조망유조우판단기대방료적민감성，가이작위예후적지표。
Objective:The aims of this study are to detect the apoptosis of cancer cells after a single dose of radiotherapy with 99mTc-HYNIC-annexin V and to investigate the correlation among early apoptosis, radio-therapeutic dose, and radio-sensitivity. Methods:Ten days after respective inoculations of EL4 lymphoma and S180 sarcoma in their right upper limbs, the mice were randomly divided into imaging group (Group One) and observation group (Group Two). In Group One, 99mTc-HYNIC-annexin V was injected via the caudal vein after different doses of irradiation. Approximately 2 h later, clinical imaging was conducted by single-photon emission-computed tomography. The mice were sacrificed to evaluate the bio-distribution of 99mTc in each specimen. Cell count during apoptosis was conducted through the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. Observation was conducted in Group Two for 2 weeks after the irradiation. Results:Tumor uptake of 99mTc-HYNIC-annexin V significantly correlated with the number of TUNEL-positive cells, which concordantly increased with the increase of dosage (r=0.892, P=0.000). With the same dose (0 or 8 Gy), the values of%ID/g, T/B, T/M, and TUNEL-positive cell number of EL4 lymphoma were significantly higher compared with those of S180 sarcoma. EL4 lymphoma was entirely minimized after irradiation at 8 Gy. Conclusion: 99mTc-HYNIC-annexin V can detect early apoptosis in vivo in tumors receiving radiation. The irradiation-induced apoptosis in vivo determined with 99mTc-HYNIC-annexin V positively correlates with the curative effect of tumors. The detection of tumor cell apoptosis via 99mTc-HYNIC-annexin V helps estimate radio-sensitivity, and can become a predictive index for radiotherapy.