山西医药杂志
山西醫藥雜誌
산서의약잡지
SHANXI MEDICAL JOURNAL
2014年
7期
754-756
,共3页
陈晓锐%彭俊旭%安正红%郑华%李鹤
陳曉銳%彭俊旭%安正紅%鄭華%李鶴
진효예%팽준욱%안정홍%정화%리학
哮喘%白细胞介素-5%免疫球蛋白 E%肺功能测定%儿童
哮喘%白細胞介素-5%免疫毬蛋白 E%肺功能測定%兒童
효천%백세포개소-5%면역구단백 E%폐공능측정%인동
Asthma%Interleukin-5%Immunoglobulin E%Pulmonary function test%Children
目的：检测支气管哮喘患儿外周血白细胞介素（IL）-5、总 IgE（TIgE）和肺功能水平，探讨 IL-5、TIgE 与肺功能水平及支气管哮喘之间的相关性。方法选取35例门诊随诊的哮喘缓解期患儿作为哮喘组，30名同期健康体检儿童作为健康对照组，2组儿童均于清晨空腹取外周静脉血1.0 mL 制备血清，应用双抗体夹心酶联免疫吸附试验（ELISA）法测定2组儿童血清 IL-5、TIgE，并行肺功能检测。结果哮喘组患儿血清 IL-5、TIgE 水平均较健康对照组升高，差异有统计学意义（t ＝6.84、21.63，P ＜0.01），肺功能用力呼出25％肺活量时呼气流量（FEF25％）、用力呼出50％肺活量时呼气流量（FEF50％）哮喘组较健康对照组水平明显下降（t ＝-2.258、-2.428，P ＜0.05），而第1秒用力呼气容积占用力肺活量的比值（FEV1／FVC％）差异无统计学意义；IL-5与 FEV1／FVC％、FEF25％、FEF50％无相关性，TIgE 与 FEV1／FVC％、FEF25％、FEF50％呈负相关（r＝-0.534、-0.392、-0.394，P ＜0.05）。结论哮喘患儿体内存在 Th1／Th2免疫失衡，IL-5和 TIgE 参与气道高反应与呼吸道重塑的病理生理过程，TIgE 即使在哮喘缓解期也仍然和肺功能密切相关，可以成为辅助判定哮喘严重程度的参考指标。
目的：檢測支氣管哮喘患兒外週血白細胞介素（IL）-5、總 IgE（TIgE）和肺功能水平，探討 IL-5、TIgE 與肺功能水平及支氣管哮喘之間的相關性。方法選取35例門診隨診的哮喘緩解期患兒作為哮喘組，30名同期健康體檢兒童作為健康對照組，2組兒童均于清晨空腹取外週靜脈血1.0 mL 製備血清，應用雙抗體夾心酶聯免疫吸附試驗（ELISA）法測定2組兒童血清 IL-5、TIgE，併行肺功能檢測。結果哮喘組患兒血清 IL-5、TIgE 水平均較健康對照組升高，差異有統計學意義（t ＝6.84、21.63，P ＜0.01），肺功能用力呼齣25％肺活量時呼氣流量（FEF25％）、用力呼齣50％肺活量時呼氣流量（FEF50％）哮喘組較健康對照組水平明顯下降（t ＝-2.258、-2.428，P ＜0.05），而第1秒用力呼氣容積佔用力肺活量的比值（FEV1／FVC％）差異無統計學意義；IL-5與 FEV1／FVC％、FEF25％、FEF50％無相關性，TIgE 與 FEV1／FVC％、FEF25％、FEF50％呈負相關（r＝-0.534、-0.392、-0.394，P ＜0.05）。結論哮喘患兒體內存在 Th1／Th2免疫失衡，IL-5和 TIgE 參與氣道高反應與呼吸道重塑的病理生理過程，TIgE 即使在哮喘緩解期也仍然和肺功能密切相關，可以成為輔助判定哮喘嚴重程度的參攷指標。
목적：검측지기관효천환인외주혈백세포개소（IL）-5、총 IgE（TIgE）화폐공능수평，탐토 IL-5、TIgE 여폐공능수평급지기관효천지간적상관성。방법선취35례문진수진적효천완해기환인작위효천조，30명동기건강체검인동작위건강대조조，2조인동균우청신공복취외주정맥혈1.0 mL 제비혈청，응용쌍항체협심매련면역흡부시험（ELISA）법측정2조인동혈청 IL-5、TIgE，병행폐공능검측。결과효천조환인혈청 IL-5、TIgE 수평균교건강대조조승고，차이유통계학의의（t ＝6.84、21.63，P ＜0.01），폐공능용력호출25％폐활량시호기류량（FEF25％）、용력호출50％폐활량시호기류량（FEF50％）효천조교건강대조조수평명현하강（t ＝-2.258、-2.428，P ＜0.05），이제1초용력호기용적점용력폐활량적비치（FEV1／FVC％）차이무통계학의의；IL-5여 FEV1／FVC％、FEF25％、FEF50％무상관성，TIgE 여 FEV1／FVC％、FEF25％、FEF50％정부상관（r＝-0.534、-0.392、-0.394，P ＜0.05）。결론효천환인체내존재 Th1／Th2면역실형，IL-5화 TIgE 삼여기도고반응여호흡도중소적병리생리과정，TIgE 즉사재효천완해기야잉연화폐공능밀절상관，가이성위보조판정효천엄중정도적삼고지표。
Objective To explore the changes of levels of interleukin-5 (IL-5),total IgE (TIgE)and pulmonary function test in asthmatic children,and to study on the correlation between the level of these factors and the level of pul-monary function.Methods Thirty-five children with asthma (clinical paracmasis)were selected as asthmatic group,30 healthy children were selected as healthy control group.All the children were drawn 1.0 mL peripheral vein blood in the early morning with empty stomach to prepare the serum.Then the contents of IL-5 and TIgE of serum in asthmatic group and healthy control group were detected by enzyme-linked immunosorbent assay(ELISA)and pulmonary function was test-ed simultaneously.Results The levels of IL-5 and TIgE of serum in asthmatic children were higher significantly than those in healthy control group (t=6.84,21.63,P <0.01).The level of FEF25% and FEF50% in asthmatic children were lower than those in healthy control group (t=-2.258,-2.42,P <0.05).For FEV1/FVC% in two groups, there was no significant difference.There was no correlation between IL-5 levels in asthmatic children and lung function (FEV1/FVC%,FEF25%,FEF50%),while TIgE level and lung function(FEV1/FVC%,FEF25%,FEF50%)levels was positively correlated (r=-0.534,-0.392,-0.394,P<0.05).Conclusion Asthmatic children have an imbalance of Th1/Th2 cell immunity,IL-5 and TIgE were involved in the pathophysiological process of airway hyperresponsiveness and airway remodeling.TIgE is closely related to pulmonary function even in asthma remission,which can become effec-tive reference index of auxiliary decision of the severity of asthma.
