中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
2期
100-103
,共4页
王昊%路小婷%贾志健%牛侨
王昊%路小婷%賈誌健%牛僑
왕호%로소정%가지건%우교
铝%tau蛋白%磷酸化%神经毒性
鋁%tau蛋白%燐痠化%神經毒性
려%tau단백%린산화%신경독성
Aluminum%Tau protein%Phosphorylation%Neurotoxicity
目的 探讨三氯化铝对SH-SY5Y细胞及tau蛋白异常磷酸化水平的影响.方法 选取SH-SY5Y细胞进行三氯化铝染毒,实验分为对照组、200、400和800μmol/L Al3+染毒组,染毒48 h后观察细胞形态学变化,采用CCK-8法测定细胞活性,并提取细胞蛋白,采用蛋白免疫印迹法(Western blot)检测细胞蛋白P-tau181、231、262、396和tau5的表达情况.结果 随着染铝浓度增加细胞数目减少,突触回缩,细胞活性测定结果显示,800 μmol/L A13+组细胞活性明显低于对照组,差异有统计学意义(P＜0.05);Western-Blot结果显示200、400和800 μmol/L Al3+组P-taul81、231、396和tau5蛋白的表达均明显高于对照组,差异有统计学意义(P＜0.05),800μmol/l.Al3+组P-tau262蛋白表达明显高于对照组,差异亦有统计学意义(P＜0.05).结论 在本实验条件下,三氯化铝对SH-SY5Y细胞有毒性作用,低浓度就可引起tau181、231、396和tau5表达异常.
目的 探討三氯化鋁對SH-SY5Y細胞及tau蛋白異常燐痠化水平的影響.方法 選取SH-SY5Y細胞進行三氯化鋁染毒,實驗分為對照組、200、400和800μmol/L Al3+染毒組,染毒48 h後觀察細胞形態學變化,採用CCK-8法測定細胞活性,併提取細胞蛋白,採用蛋白免疫印跡法(Western blot)檢測細胞蛋白P-tau181、231、262、396和tau5的錶達情況.結果 隨著染鋁濃度增加細胞數目減少,突觸迴縮,細胞活性測定結果顯示,800 μmol/L A13+組細胞活性明顯低于對照組,差異有統計學意義(P＜0.05);Western-Blot結果顯示200、400和800 μmol/L Al3+組P-taul81、231、396和tau5蛋白的錶達均明顯高于對照組,差異有統計學意義(P＜0.05),800μmol/l.Al3+組P-tau262蛋白錶達明顯高于對照組,差異亦有統計學意義(P＜0.05).結論 在本實驗條件下,三氯化鋁對SH-SY5Y細胞有毒性作用,低濃度就可引起tau181、231、396和tau5錶達異常.
목적 탐토삼록화려대SH-SY5Y세포급tau단백이상린산화수평적영향.방법 선취SH-SY5Y세포진행삼록화려염독,실험분위대조조、200、400화800μmol/L Al3+염독조,염독48 h후관찰세포형태학변화,채용CCK-8법측정세포활성,병제취세포단백,채용단백면역인적법(Western blot)검측세포단백P-tau181、231、262、396화tau5적표체정황.결과 수착염려농도증가세포수목감소,돌촉회축,세포활성측정결과현시,800 μmol/L A13+조세포활성명현저우대조조,차이유통계학의의(P＜0.05);Western-Blot결과현시200、400화800 μmol/L Al3+조P-taul81、231、396화tau5단백적표체균명현고우대조조,차이유통계학의의(P＜0.05),800μmol/l.Al3+조P-tau262단백표체명현고우대조조,차이역유통계학의의(P＜0.05).결론 재본실험조건하,삼록화려대SH-SY5Y세포유독성작용,저농도취가인기tau181、231、396화tau5표체이상.
Objective To investigate the effect of aluminum trichloride on the abnormal phosphorylation of tau protein in SH-SY5Y cells.Methods SH-SY5Y cells were assigned to control group and aluminum trichloride exposure groups (200,400,and 800 μmol/L Al3+).The cell morphology was observed after 48 hours of exposure; the cell viability was measured by CCK-8 assay; total protein was extracted from the cells,and the expression of phospho-tau (p-tau) 181,231,262,and 396 and tau 5 was measured by Western blot.Results As the Al3+ concentration rose,the number of living SH-SY5Y cells decreased,and the synapses of the cells retracted.The viability of cells exposed to 800 μmol/L A13+ was significantly lower than that of the control group (P＜0.05).The 200,400,and 800 μmol/L Al3+ exposure groups showed significantly higher expression of p-tau 181,231,and 396 and tau 5 than the control group (P＜0.05),and the 800 μmol/L Al3+ exposure group showed significantly higher expression of p-tau 262 than the control group (P＜0.05).Conclusion Under the present experimental conditions,aluminum trichloride has toxic effect on SH-SY5Y cells and can lead to abnormal expression of p-tau 181,231,and 396 and tau 5 at low Al3+ concentration.
