CAJ | 학술논문

在已构建好的蜡梅花 cDNA文库及 EST分析的基础上,随机克隆测序得到一个蜡梅 FKBP 基因,将其命名为CpFKBP,Genebank登录号：JQ337962.该基因cDNA长为482 bp,具有一个339 bp的ORF,编码一个113个氨基酸的多肽,预测等电点为8.48,理论分子量为12.08 KD.在大肠杆菌中获得并纯化了重组的 CpFKBP 蛋白质,并进一步对重组蛋白进行PPIase活性分析,结果显示CpFKBP重组蛋白有较高的PPIase酶活性.
재이구건호적사매화 cDNA문고급 EST분석적기출상,수궤극륭측서득도일개사매 FKBP 기인,장기명명위CpFKBP,Genebank등록호：JQ337962.해기인cDNA장위482 bp,구유일개339 bp적ORF,편마일개113개안기산적다태,예측등전점위8.48,이론분자량위12.08 KD.재대장간균중획득병순화료중조적 CpFKBP 단백질,병진일보대중조단백진행PPIase활성분석,결과현시CpFKBP중조단백유교고적PPIase매활성.
A FKBP gene has been obtained by randomly sequencing the cDNA library of Chimonanthus praecox flowers,which is named as CpFKBP (GenBank accession No:JQ337962).The 482 bp cDNA of CpFKBP contains a 339 bp open reading frame (ORF)encoding a 113-amino-acid polypeptide with a cal-culated molecular mass of 12.08 KD and the theoretical pI of 8.48.The recombinant CpFKBP with the fused TRX and His Tags at it N-terminus has been purified by the His-binding purification Kit.The analy-sis of PPIase activity shows that the fusion protein of CpFKBP has a high PPIase activity.This study has facilitated the research of the FKBP genes in different species,the application of immuno-suppressants and understanding the whole immunophilin family.