中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2013年
10期
1441-1443
,共3页
促红细胞生成素%血管紧张素受体拮抗剂%再灌注损伤
促紅細胞生成素%血管緊張素受體拮抗劑%再灌註損傷
촉홍세포생성소%혈관긴장소수체길항제%재관주손상
Erythropoietin%Angiotensin receptor blocker%Reperfusion injury
目的 研究促红细胞生成素(EPO)和血管紧张素受体拮抗剂对脑缺血后内皮型一氧化氮合酶(eNOS)和凋亡相关蛋白Bcl-2表达的影响.方法 采用健康S-D大鼠,阻断一侧大脑中动脉血流2 h再灌注24 h建立局灶性脑缺血再灌注损伤模型(MCAO),治疗组分别于缺血再灌注前后经腹腔注射EPO3 000 u·kg-1·d-1或缬沙坦40 mg·kg-1·d-1,检测eNOS、Bcl-2的表达.结果 与缺血再灌注组相比,EPO处理组和联合使用EPO、血管紧张素受体拮抗剂组eNOS、Bcl-2蛋白表达增高(P＜0.05),血管紧张素受体拮抗剂处理组eNOS表达升高(P＜0.05),Bcl-2蛋白表达有升高,但差异无统计学意义.结论 EPO可上调Bcl-2蛋白、eNOS表达而实现脑保护作用,血管紧张素受体拮抗剂可上调eNOS表达而实现脑保护作用.
目的 研究促紅細胞生成素(EPO)和血管緊張素受體拮抗劑對腦缺血後內皮型一氧化氮閤酶(eNOS)和凋亡相關蛋白Bcl-2錶達的影響.方法 採用健康S-D大鼠,阻斷一側大腦中動脈血流2 h再灌註24 h建立跼竈性腦缺血再灌註損傷模型(MCAO),治療組分彆于缺血再灌註前後經腹腔註射EPO3 000 u·kg-1·d-1或纈沙坦40 mg·kg-1·d-1,檢測eNOS、Bcl-2的錶達.結果 與缺血再灌註組相比,EPO處理組和聯閤使用EPO、血管緊張素受體拮抗劑組eNOS、Bcl-2蛋白錶達增高(P＜0.05),血管緊張素受體拮抗劑處理組eNOS錶達升高(P＜0.05),Bcl-2蛋白錶達有升高,但差異無統計學意義.結論 EPO可上調Bcl-2蛋白、eNOS錶達而實現腦保護作用,血管緊張素受體拮抗劑可上調eNOS錶達而實現腦保護作用.
목적 연구촉홍세포생성소(EPO)화혈관긴장소수체길항제대뇌결혈후내피형일양화담합매(eNOS)화조망상관단백Bcl-2표체적영향.방법 채용건강S-D대서,조단일측대뇌중동맥혈류2 h재관주24 h건립국조성뇌결혈재관주손상모형(MCAO),치료조분별우결혈재관주전후경복강주사EPO3 000 u·kg-1·d-1혹힐사탄40 mg·kg-1·d-1,검측eNOS、Bcl-2적표체.결과 여결혈재관주조상비,EPO처리조화연합사용EPO、혈관긴장소수체길항제조eNOS、Bcl-2단백표체증고(P＜0.05),혈관긴장소수체길항제처리조eNOS표체승고(P＜0.05),Bcl-2단백표체유승고,단차이무통계학의의.결론 EPO가상조Bcl-2단백、eNOS표체이실현뇌보호작용,혈관긴장소수체길항제가상조eNOS표체이실현뇌보호작용.
Objective To study the effects of erythropoietin(EPO) and angiotensin receptor blocker on the expression of eNOS and Bcl-2 after cerebral ischemia.Methods The model of focal cerebral ischemia was made by occluding middle cerebral artery(MCA) for 2h and reperfusing for 48h in healthy Sprague-Dawley rats.The rats received EPO 3 000u · kg-1 · d-1 or valsartan 40mg · kg-1 · d-1 by intraperitoneal injection just before the beginning of reperfusion and after reperfusion.The expression of eNOS and Bcl-2 was detected.Results Compared with ischemia group,Bcl-2 and eNOS expressions in EPO-treated group and EPO-ARB treated group were significantly increased (P ＜ 0.05).eNOS expression in ARB-treated group was significantly increased (P ＜ 0.05),Bcl-2 expression was up-regulated,but it had no significant difference.Conclusion EPO had protective effects on cerebral ischemia-reperfu-sion injury in rats,which partially mediated by up-regulating of Bcl-2 and eNOS expression.Angiotensin receptor blocker had protective effects on cerebral ischemia-reperfusion injury in rats,which partially mediated by up-regulating of eNOS expression.
