CAJ | 학술논문

目的观察大黄素对非酒精性脂肪肝大鼠胰岛素抵抗、瘦素的作用,探讨其防治脂肪肝的可能机制.方法将42只SD大鼠按体重大小编号后采用随机数字表法分为2组:正常组(A组、8只),给予普通饲料喂养；高脂饲料喂养组(M组、34只),给予高脂饲料喂养.于4周末验证有脂肪肝后,M组32只大鼠按体重大小编号后用随机数字表法分为M1、M2、M3、M4四个亚组,每组8只,各大鼠饲养饲料不变,其中,M2、M3、M4组分别予低剂量大黄素、高剂量大黄素、二甲双胍干预,大黄素、二甲双胍以0.5％羧甲基纤维素钠溶解,A组、M1组予0.5％羧甲基纤维素钠灌胃.8周末测定血清瘦素、稳态模式评估法胰岛素抵抗指数(HOMA-IR)、胰岛素敏感指数(ISI),评价肝脂肪变和炎症程度.结果与M1组比较,低、高剂量大黄素均能改善由HOMA-IR和ISI所评价的胰岛素抵抗(P ＜0.05,P＜0.01).M1组血清瘦素较A组升高(P＜0.01),M2、M3组血清瘦素较M1组降低(P ＜0.05,P＜0.01)；M1组血清瘦素水平与HOMA-IR呈正相关(r=0.746,P＜0.05),与ISI呈负相关(r=-0.731,P＜0.05).与M1组比较,低、高剂量大黄素对大鼠肝脏脂肪变有不同改善(P ＜0.05,P＜0,01),而对肝脏炎症均有改善(P＜0.01).结论大黄素降低血清瘦素水平,改善胰岛素抵抗,可能是其防治大鼠非酒精性脂肪肝的重要机制之一.
목적 관찰대황소대비주정성지방간대서이도소저항、수소적작용,탐토기방치지방간적가능궤제.방법 장42지SD대서안체중대소편호후채용수궤수자표법분위2조:정상조(A조、8지),급여보통사료위양；고지사료위양조(M조、34지),급여고지사료위양.우4주말험증유지방간후,M조32지대서안체중대소편호후용수궤수자표법분위M1、M2、M3、M4사개아조,매조8지,각대서사양사료불변,기중,M2、M3、M4조분별여저제량대황소、고제량대황소、이갑쌍고간예,대황소、이갑쌍고이0.5％최갑기섬유소납용해,A조、M1조여0.5％최갑기섬유소납관위.8주말측정혈청수소、은태모식평고법이도소저항지수(HOMA-IR)、이도소민감지수(ISI),평개간지방변화염증정도.결과 여M1조비교,저、고제량대황소균능개선유HOMA-IR화ISI소평개적이도소저항(P ＜0.05,P＜0.01).M1조혈청수소교A조승고(P＜0.01),M2、M3조혈청수소교M1조강저(P ＜0.05,P＜0.01)；M1조혈청수소수평여HOMA-IR정정상관(r=0.746,P＜0.05),여ISI정부상관(r=-0.731,P＜0.05).여M1조비교,저、고제량대황소대대서간장지방변유불동개선(P ＜0.05,P＜0,01),이대간장염증균유개선(P＜0.01).결론 대황소강저혈청수소수평,개선이도소저항,가능시기방치대서비주정성지방간적중요궤제지일.
Objective To investigate the effect of emodin on insulin resistance and leptin in rat with nonalcoholic fatty liver disease (NAFLD) and to explore the mechanisms of emodin treating NAFLD.Methods Forty two Spraque-Dawley rats were numbered according to their body weights,and were randomly divided into two groups(group A:8 rats; group M:34 rats) by random number table method.The rats in group A was fed with ordinary diets and Group M with improved high fat diets.Four weeks later,when hepatic steatosis in group M were identified,the remaining 32 rats in group M were numbered according to their body weights,and were divided randomly into 4 subgroups (group M1,M2,M3 and M4) by random number table method,with 8 rats in each subgroup.The feeding of all rats was unchanged.The rats in group M2,M3 and M4 were separately intervened with emodin by low doses,emodin by high doses and metformin.Emodin and metformin were dissolved by 0.5％ sodium carboxymethyl cellulose.The rats in group A and M1 was fed with 0.5％ sodium carboxymethyl cellulose by gavage.Four weeks later,all rats were executed.The serum glucose was measured with automatic biochemical analyzer.The serum insulin and leptin were measured by radioimmunoassay (RIA).Insulin resistance was estimated by insulin resistance index of homeostasis model assessment (HOMA-IR) and insulin sensitivity index (ISI).Liver biopsy tissues were treated by Hematoxylin-eosin (HE) staining to evaluate the degree of steatosis and inflammation of liver.Results Compared with group M1,the low and high dosage emodin improved insulin resistance which was represent by serum insulin,HOMA-IR,and ISI(P ＜0.05,P ＜0.01).The serum leptin in group M1 was higher than that in group A (P ＜0.01).The serum leptin in groups M2 and M3 was lower than that in group M1(P ＜0.05,P ＜0.01).Correlation analysis showed that the serum leptin was positively correlated with HOMA-IR(r =0.746,P ＜0.05),and negatively correlated with ISI(r =-0.731,P ＜ 0.05)in group M1.Compared with group M1,the low and high dosage emodin together had the respective effect of ameliorating steatosis(P ＜0.05,P ＜0.01),and they also reduced the hepatic inflammatory activity(P ＜ 0.01).Conclusions Reducing serum leptin and improving insulin resistance may be the mechanisms of emodin treating NAFLD.