中国药物应用与监测
中國藥物應用與鑑測
중국약물응용여감측
CHINESE JOURNAL OF DRUG APPLICATION AND MONITORING
2014年
2期
92-94
,共3页
万古霉素%去甲万古霉素%高效液相色谱法
萬古黴素%去甲萬古黴素%高效液相色譜法
만고매소%거갑만고매소%고효액상색보법
Vancomycin%Norvancomycin%HPLC
目的：建立同时测定血清中万古霉素及去甲万古霉素浓度的高效液相色谱法。方法：采用Diamonsil C18色谱柱（250 mm×4.6 mm，5μm）；流动相：乙腈-磷酸二氢钾缓冲液（pH=3.2）（7.5∶92.5）；流速：1 mL·min-1；检测波长：236 nm；柱温：30℃；进样量：25μL；二极管阵列检测。内标法定量，万古霉素与去甲万古霉素互为内标。结果：去甲万古霉素低、中、高3个浓度（3.125、25.000、50.000μg·mL-1）的方法回收率分别为98.5%、102.2%、103.1%，万古霉素低、中、高3个浓度（3.125、25.000、50.000μg·mL-1）的方法回收率分别为96.2%、100.8%、103.5%，日内精密度RSD小于3.24%，日间精密度RSD小于6.12%。结论：本方法准确度高，灵敏度好，操作简便，符合万古霉素及去甲万古霉素血药浓度监测和药代动力学研究的要求。
目的：建立同時測定血清中萬古黴素及去甲萬古黴素濃度的高效液相色譜法。方法：採用Diamonsil C18色譜柱（250 mm×4.6 mm，5μm）；流動相：乙腈-燐痠二氫鉀緩遲液（pH=3.2）（7.5∶92.5）；流速：1 mL·min-1；檢測波長：236 nm；柱溫：30℃；進樣量：25μL；二極管陣列檢測。內標法定量，萬古黴素與去甲萬古黴素互為內標。結果：去甲萬古黴素低、中、高3箇濃度（3.125、25.000、50.000μg·mL-1）的方法迴收率分彆為98.5%、102.2%、103.1%，萬古黴素低、中、高3箇濃度（3.125、25.000、50.000μg·mL-1）的方法迴收率分彆為96.2%、100.8%、103.5%，日內精密度RSD小于3.24%，日間精密度RSD小于6.12%。結論：本方法準確度高，靈敏度好，操作簡便，符閤萬古黴素及去甲萬古黴素血藥濃度鑑測和藥代動力學研究的要求。
목적：건립동시측정혈청중만고매소급거갑만고매소농도적고효액상색보법。방법：채용Diamonsil C18색보주（250 mm×4.6 mm，5μm）；류동상：을정-린산이경갑완충액（pH=3.2）（7.5∶92.5）；류속：1 mL·min-1；검측파장：236 nm；주온：30℃；진양량：25μL；이겁관진렬검측。내표법정량，만고매소여거갑만고매소호위내표。결과：거갑만고매소저、중、고3개농도（3.125、25.000、50.000μg·mL-1）적방법회수솔분별위98.5%、102.2%、103.1%，만고매소저、중、고3개농도（3.125、25.000、50.000μg·mL-1）적방법회수솔분별위96.2%、100.8%、103.5%，일내정밀도RSD소우3.24%，일간정밀도RSD소우6.12%。결론：본방법준학도고，령민도호，조작간편，부합만고매소급거갑만고매소혈약농도감측화약대동역학연구적요구。
Objective: To establish the HPLC method for fast, effective, simultaneous determination of vancomycin and norvancomycin in serum. Methods:The extract was separated by Diamonsil C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-potassium dihydrogen phosphate buffer solution (pH=3.2) (7.5∶92.5) with the lfow rate of 1 mL·min-1. The detection wavelength was set at 236 nm. The column temperature was 30℃and the sample size was 25μL. Vancomycin and norvancomycin were used as the internal standard for each other. Results: For norvancomycin, the average method recovery rates of three concentrations (3.125, 25.000, 50.000μg·mL-1) were 98.5%, 102.2%, and 103.1%respectively. For vancomycin, the average method recovery rates of three concentrations (3.125, 25.000, 50.000μg·mL-1) were 96.2%, 100.8%and 103.5% respectively. The within-day RSD was less than 3.24%, and between-day RSD was less than 6.12%. Conclusion:The method was accurate, sensitive, simple, which was suitable for the therapeutic drug monitoring (TDM) and pharmacokinetic study of vancomycin as well as norvancomycin.
