CAJ | 학술논문

目的探讨低频电刺激(LFS)对脑梗死大鼠神经功能和梗死侧脑组织神经干细胞(NSC)增殖、血管再生的影响。方法制作大鼠永久性大脑中动脉梗死模型(MCAO)，随机分为假手术组、对照组和实验组，每组又分为7 d和14 d两个亚组，每个亚组12只。术后2 d开始进行LFS治疗。采用大鼠神经功能缺损评分(NSS)评估大鼠神经功能缺损程度，运用免疫荧光染色检测大鼠梗死侧大脑侧脑室的室管膜下层(SVZ)的5-溴尿嘧啶脱氧核糖核苷酸(BrdU)，应用酶联免疫吸附测定法(ELISA)检测大鼠梗死侧大脑的基质细胞衍生因子-1(SDF-1)、血管内皮生长因子(VEGF)含量。结果 LFS治疗后14 d，实验组大鼠NSS评分明显低于对照组和假手术组(P<0.01)；LFS治疗后各时间点实验组大鼠梗死侧大脑的SDF-1、BrdU和VEGF水平均较对照组有不同程度的上调(P<0.01)。结论 LFS能改善脑梗死大鼠功能障碍，其可能机制是通过激活SDF-1/CXCR4轴，进而发挥促进NSC增殖和血管再生作用。
목적탐토저빈전자격(LFS)대뇌경사대서신경공능화경사측뇌조직신경간세포(NSC)증식、혈관재생적영향。방법제작대서영구성대뇌중동맥경사모형(MCAO)，수궤분위가수술조、대조조화실험조，매조우분위7 d화14 d량개아조，매개아조12지。술후2 d개시진행LFS치료。채용대서신경공능결손평분(NSS)평고대서신경공능결손정도，운용면역형광염색검측대서경사측대뇌측뇌실적실관막하층(SVZ)적5-추뇨밀정탈양핵당핵감산(BrdU)，응용매련면역흡부측정법(ELISA)검측대서경사측대뇌적기질세포연생인자-1(SDF-1)、혈관내피생장인자(VEGF)함량。결과 LFS치료후14 d，실험조대서NSS평분명현저우대조조화가수술조(P<0.01)；LFS치료후각시간점실험조대서경사측대뇌적SDF-1、BrdU화VEGF수평균교대조조유불동정도적상조(P<0.01)。결론 LFS능개선뇌경사대서공능장애，기가능궤제시통과격활SDF-1/CXCR4축，진이발휘촉진NSC증식화혈관재생작용。
Objective To investigate the effects of low frequency stimulation (LFS) on proliferation and angiogenesis of neural stem cells (NSC) in cerebral infarction side in rats. Methods The rats with permanent middle cerebral artery occlusion (MCAO) were randomly divided into sham-operation group, control group and LFS group, each group was divided into 7 days and 14 days subgroups with 12 rats in each subgroup. LFS therapy was started 2 days after operation. The degree of nerve function defect was evaluated with Neurological Severi-ty Score (NSS), and the 5-bromodeoxy-dine (BrdU) positive cells in the subventricular zone (SVZ) of cerebral infarction side were detected with immunofluorescence. Stromal cell-derived factor 1(SDF-1) and vascular endothelial growth factor (VEGF) in infarction side were de-tected with enzyme-linked immunosorbent assay (ELISA). Results The NSS score was lower in the LFS group than in the control group and the sham-operation group 14 days after surgery (P<0.01). The number of BrdU positive cells, the content of SDF-1 and VEGF in the isch-emic side were more in the LFS group than in the other groups (P<0.01) after treatment. Conclusion LFS can improve the neurological func-tion in rats with acute cerebral infarction, which may associate with activating SDF-1/CXCR4 axis.