CAJ | 학술논문

目的：研究0.25 T、0.35 T、0.42 T静磁场持续或间歇曝磁对骨髓间充质干细胞(MSCs)增殖及骨向分化的影响。方法全骨髓贴壁筛选法分离MSCs，取第三代MSCs采用强度分别为0.25 T、0.35 T、0.42 T静磁场持续曝磁或间歇曝磁，CCK-8法检测各组细胞增殖活力，并观察0.35 T连续曝磁后MSCs碱性磷酸酶活性、骨钙素含量。结果细胞经过磁场处理后，与对照组相比，细胞增殖活力均降低，连续曝磁第7天效果最为显著(P<0.001)，而0.25 T、0.35 T间歇曝磁第2~8天持续表现出显著抑制效应(P<0.001)。0.35 T持续曝磁后，MSCs碱性磷酸酶活性和骨钙素水平均增高(P<0.05)。结论0.25 T、0.35 T、0.42 T静磁场连续曝磁或间歇曝磁均可抑制MSCs增殖，0.35 T连续曝磁能促进MSCs向成骨细胞方向分化。
목적：연구0.25 T、0.35 T、0.42 T정자장지속혹간헐폭자대골수간충질간세포(MSCs)증식급골향분화적영향。방법전골수첩벽사선법분리MSCs，취제삼대MSCs채용강도분별위0.25 T、0.35 T、0.42 T정자장지속폭자혹간헐폭자，CCK-8법검측각조세포증식활력，병관찰0.35 T련속폭자후MSCs감성린산매활성、골개소함량。결과세포경과자장처리후，여대조조상비，세포증식활력균강저，련속폭자제7천효과최위현저(P<0.001)，이0.25 T、0.35 T간헐폭자제2~8천지속표현출현저억제효응(P<0.001)。0.35 T지속폭자후，MSCs감성린산매활성화골개소수평균증고(P<0.05)。결론0.25 T、0.35 T、0.42 T정자장련속폭자혹간헐폭자균가억제MSCs증식，0.35 T련속폭자능촉진MSCs향성골세포방향분화。
Objective To investigate the effect of exposure to 0.25 T, 0.35 T, 0.42 T static magnetic fields (SMF) on the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs). Methods Primary bone marrow MSCs were obtained from Sprague-Dawley rats and screened by adhesive method. MSCs were exposed to 0.25 T, 0.35 T, 0.42 T SMF continuously and 24 h intermittently respectively. The cell proliferation activity was detected by Cell Counting Kit (CCK-8) assay. The osteogenic differentiation markers including alkaline phosphatase (ALP) activity and osteocalcin were analyzed after continuously exposure to 0.35 T SMF. Results Compared with the control group, the proliferation activity of SMF-treated cells significantly decreased, especially on the 7th day (P<0.001) after continuous exposure, and on the 2nd to 8th day in 0.25 T, 0.35 T SMF intermittent exposure groups (P<0.001). Both the alkaline phosphatase activity and the lev-el of osteocalcin significantly increased in MSCs after continuous exposure to 0.35 T SMF (P<0.05). Conclusion Continuous or intermittent exposure to 0.25 T, 0.35 T and 0.42 T SMF could effectively inhibit the proliferation of MSCs. Continuous exposure to 0.35 T SMF could enhance the osteogenic differentiation of MSCs.