天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2014年
4期
318-320
,共3页
万晨光%冯学泉%李牧%杜洪印%张玮晔%史源%刘蕾%沈中阳
萬晨光%馮學泉%李牧%杜洪印%張瑋曄%史源%劉蕾%瀋中暘
만신광%풍학천%리목%두홍인%장위엽%사원%류뢰%침중양
脑死亡%颅内压%模型,动物%猪%硬膜外颅内加压
腦死亡%顱內壓%模型,動物%豬%硬膜外顱內加壓
뇌사망%로내압%모형,동물%저%경막외로내가압
brain death%intracranial pressure%models,animal%swine%epidural intracranial pressure
目的：建立一种稳定、可靠的猪脑死亡模型，为研究脑死亡模型建立过程中、脑死亡判定后的脑组织病理改变及器官移植免疫等变化提供一个更加稳定的动物模型。方法以经典的硬膜外颅内加压法为基础，在长白猪颅内置入Codman有创颅内压监测探头，在实时监测颅内压变化情况下，根据颅内压与平均动脉压（MAP）的关系进行间断颅内加压建立脑死亡模型。结果12例实验用长白猪，1头死于麻醉意外，余11头均成功诱导脑死亡模型。经有效呼吸循环支持可维持脑死亡模型（31.3±4.7）h。通过经颅多普勒、脑电图、心电、MAP等监测显示，脑死亡模型制作过程中各参数变化平稳，脑死亡判定后，动物模型可长时间维持。结论在颅内压监测条件下建立猪脑死亡动脉模型的方法稳定可靠，易于标准化，可为脑死亡模型建立过程中及脑死亡判定后的脑功能及器官移植免疫等研究提供更加稳定的载体。
目的：建立一種穩定、可靠的豬腦死亡模型，為研究腦死亡模型建立過程中、腦死亡判定後的腦組織病理改變及器官移植免疫等變化提供一箇更加穩定的動物模型。方法以經典的硬膜外顱內加壓法為基礎，在長白豬顱內置入Codman有創顱內壓鑑測探頭，在實時鑑測顱內壓變化情況下，根據顱內壓與平均動脈壓（MAP）的關繫進行間斷顱內加壓建立腦死亡模型。結果12例實驗用長白豬，1頭死于痳醉意外，餘11頭均成功誘導腦死亡模型。經有效呼吸循環支持可維持腦死亡模型（31.3±4.7）h。通過經顱多普勒、腦電圖、心電、MAP等鑑測顯示，腦死亡模型製作過程中各參數變化平穩，腦死亡判定後，動物模型可長時間維持。結論在顱內壓鑑測條件下建立豬腦死亡動脈模型的方法穩定可靠，易于標準化，可為腦死亡模型建立過程中及腦死亡判定後的腦功能及器官移植免疫等研究提供更加穩定的載體。
목적：건립일충은정、가고적저뇌사망모형，위연구뇌사망모형건립과정중、뇌사망판정후적뇌조직병리개변급기관이식면역등변화제공일개경가은정적동물모형。방법이경전적경막외로내가압법위기출，재장백저로내치입Codman유창로내압감측탐두，재실시감측로내압변화정황하，근거로내압여평균동맥압（MAP）적관계진행간단로내가압건립뇌사망모형。결과12례실험용장백저，1두사우마취의외，여11두균성공유도뇌사망모형。경유효호흡순배지지가유지뇌사망모형（31.3±4.7）h。통과경로다보륵、뇌전도、심전、MAP등감측현시，뇌사망모형제작과정중각삼수변화평은，뇌사망판정후，동물모형가장시간유지。결론재로내압감측조건하건립저뇌사망동맥모형적방법은정가고，역우표준화，가위뇌사망모형건립과정중급뇌사망판정후적뇌공능급기관이식면역등연구제공경가은정적재체。
Objective To establish a stable and reliable model of brain death in swine, and to provide a more stable model for investigating pathomorphology in brain tissue and for studying transplantation immunology during brain death. Methods Base on the classic methodology of increasing epidural intracranial pressure, Codman intracranial pressure moni-toring probes were implanted in landrace pigs invasively. According to the relationship between ICP and MAP, brain death models were established by increasing intracranial pressure slowly and intermittently, with real-time monitoring of the intra-cranial pressure. Results Among twelve experimental landrace pigs, one died from anesthetic accident, while the rest elev-en were successfully established as brain death models. With effective respiratory and circulatory support, those brain death models can be maintained for (31.3 ± 4.7) h. Brain death model establishement is a stable and reliable process demonstrated by transcranial Doppler, EEG, ECG, mean arterial blood pressure and other monitoring methods. After brain death is con-firmed, animal models can be maintained for a long time. Conclusion Our methodology of inducing brain death model un-der ICP monitoring is stable and easy to be standardized. It can also provide a more stable model for studying brain tissue pathomorphology and transplantation immunology.
