CAJ | 학술논문

目的：探讨周细胞在子宫内膜异位症（EMs）血管生成中的作用。方法采用免疫组化方法（SP法）检测60例EMs患者（EMs组）在位内膜（增殖期、分泌期内膜各20例）、异位内膜20例及40例非子宫内膜异位症患者（对照组）子宫内膜（增殖期、分泌期各20例），分别选用平滑肌肌动蛋白α（α-SMA）标记平均周细胞数（PN）和CD34标记微血管密度（MVD），并对二者比值（PN/MVD）进行比较，分析周细胞与EMs血管生成之间的关系。结果 EMs组异位内膜MVD高于正常增殖期和正常分泌期内膜（P<0.05），在位内膜增殖期、分泌期的MVD与相应的正常内膜比较差异均无统计学意义，在位内膜及正常内膜组内比较均为分泌期高于增殖期（P<0.05）。EMs组异位内膜PN低于正常增殖期内膜（P<0.05），与正常分泌期内膜相比差异无统计学意义，在位内膜增殖期、分泌期的PN与相应的正常内膜相比差异均无统计学意义，组内比较均分泌期低于增殖期（P<0.05）；EMs组异位内膜的PN/MVD低于正常增殖期和正常分泌期内膜（P<0.05）。在位内膜无论增殖期还是分泌期的PN/MVD与相应的正常内膜比较，均为在位内膜低于正常内膜（P<0.05），组内比较均分泌期低于增殖期（P<0.05）。结论周细胞对EMs血管生成起着重要作用，周细胞覆盖率是判定子宫内膜血管成熟度和稳定性的重要指标。
목적：탐토주세포재자궁내막이위증（EMs）혈관생성중적작용。방법채용면역조화방법（SP법）검측60례EMs환자（EMs조）재위내막（증식기、분비기내막각20례）、이위내막20례급40례비자궁내막이위증환자（대조조）자궁내막（증식기、분비기각20례），분별선용평활기기동단백α（α-SMA）표기평균주세포수（PN）화CD34표기미혈관밀도（MVD），병대이자비치（PN/MVD）진행비교，분석주세포여EMs혈관생성지간적관계。결과 EMs조이위내막MVD고우정상증식기화정상분비기내막（P<0.05），재위내막증식기、분비기적MVD여상응적정상내막비교차이균무통계학의의，재위내막급정상내막조내비교균위분비기고우증식기（P<0.05）。EMs조이위내막PN저우정상증식기내막（P<0.05），여정상분비기내막상비차이무통계학의의，재위내막증식기、분비기적PN여상응적정상내막상비차이균무통계학의의，조내비교균분비기저우증식기（P<0.05）；EMs조이위내막적PN/MVD저우정상증식기화정상분비기내막（P<0.05）。재위내막무론증식기환시분비기적PN/MVD여상응적정상내막비교，균위재위내막저우정상내막（P<0.05），조내비교균분비기저우증식기（P<0.05）。결론주세포대EMs혈관생성기착중요작용，주세포복개솔시판정자궁내막혈관성숙도화은정성적중요지표。
Objective To study the expression of pericyte and the association between pericyte and angiogenesis in the endometriosis. Methods the endometriosis group is consisted of 20 ectopic endometrium, 20 eutopic endometrium dur-ing proliferation phase, 20 eutopic endometrium during secretory phase of patient with endometriosis. The control group (non-endometriosis ) include 20 normal endometrium during proliferation phase and 20 normal endometrium during prolifer-ation phase of women without entometriosis. The histological morphology parameters such as mean density of microvessel (MVD), pericytes number(PN)as well as the average ratio of pericyte to endothelial cells in microvessels were measured by immunohistochemical and morphological assesses. Results The expression of MVD was lower in the normal endometrium and eutopic endometrium compared with it in ectopic endometrium tissue. The difference of the MVD among the different groups was statistical significant (P<0.05). The expression of MVD in secretory phase endometrium was higher than it in proliferative phase endometrium (P<0.05), and it shows no significant difference between eutopic endometrium and normal endometrium. The expression of PN and PN/MVD in ectopic and eutopic endometrium in EMs group were lower than those in control group. However, we also found that the difference of PN and PN/MVD was not statistically significant. Conclusion Pericytes play an important role in angiogenesis of the endometriosis ,and pericyte coverage contribute to maturing of func-tional vasculature of endometriosis.