CAJ | 학술논문

本文采用高效液相色谱-氢化物发生-原子荧光联技术( HPLC-HG-AFS)对海藻中的无机砷进行检测。以藻类中羊栖菜的无机砷含量为指标,确定提取剂的种类和浓度,并通过曲面拟合的方法确定了最佳提取温度和提取时间。结果表明,以1．0%HCL为提取剂,提取温度52℃,提取时间78 min的效果最佳。该方法无机砷的检出限为0．0628 mg/kg,样品加标量为0．2 mg/kg 和1．0 mg/kg 时回收率在80．5%~105．7%之间,相对标准偏差(RSD, n=6)在4．2%~5．3%之间。并应用此分析技术测定了福建省内60个不同产地紫菜样品中无机砷含量,结果表明,主要生产区紫菜中无机砷含量很低,仅占总砷含量的0~1．96%。
본문채용고효액상색보-경화물발생-원자형광련기술( HPLC-HG-AFS)대해조중적무궤신진행검측。이조류중양서채적무궤신함량위지표,학정제취제적충류화농도,병통과곡면의합적방법학정료최가제취온도화제취시간。결과표명,이1．0%HCL위제취제,제취온도52℃,제취시간78 min적효과최가。해방법무궤신적검출한위0．0628 mg/kg,양품가표량위0．2 mg/kg 화1．0 mg/kg 시회수솔재80．5%~105．7%지간,상대표준편차(RSD, n=6)재4．2%~5．3%지간。병응용차분석기술측정료복건성내60개불동산지자채양품중무궤신함량,결과표명,주요생산구자채중무궤신함량흔저,부점총신함량적0~1．96%。
This paper is about the research of the determination of inorganic arsenic in edible seaweeds with High performance liquid chromatography-hydride generation-atomic fluorescence spectrometry ( HPLC-HG-AFS) . The curved surface fitting method was applied to establish optimum conditions of extraction reagent, time and temperature based on the inorganic arsenic contents of Sargassum fusiforme. It was found that the op-timum conditions is with 0. 1%HCL at 52℃ for 78 min. The detection limit of methodology was 0. 0 628 mg/kg. The recovery rates of 1. 0 mg/kg and 10. 0 mg/kg in samples were between 80. 5% and 105. 7%, and the relative standard deviations were between 4. 5% and 5. 0%. The content of inorganic arsenic of sixty types of laver from different areas in Fujian Province was examined. The results showed that inorganic arsenic con-centration in laver was below, which accounted for the total arsenic concentration of 0~1. 96%.