安徽化工
安徽化工
안휘화공
ANHUI CHEMICAL INDUSTRY
2014年
2期
74-75,81
,共3页
董秀丽%张文成%任少伟%李兵%李如福%李学彬%李广龙
董秀麗%張文成%任少偉%李兵%李如福%李學彬%李廣龍
동수려%장문성%임소위%리병%리여복%리학빈%리엄룡
菊花超细粉%HPLC法%绿原酸
菊花超細粉%HPLC法%綠原痠
국화초세분%HPLC법%록원산
Chrysanthemum Ultrafine powder%HPLC method%Chlorogenic acid
目的：采用HPLC法测定菊花超细粉中绿原酸的含量。方法：采用Agilent Eclipse Plus C18（5μm ,4.6×250mm）色谱柱，流动相为乙腈-0.1%磷酸水溶液（10∶90，V/V），检测波长为348nm，流速1.0mL/min，进样量20μL。结果：绿原酸54.375～870mg/L范围呈线性，相关系数R为0.9998，该方法重复性及回收率均符合要求。结论：本法用于测定菊花超细粉中绿原酸的含量测定，具有简便、准确、高效等特点，可为鉴别菊花超微粉的真伪提供一定科学依据。
目的：採用HPLC法測定菊花超細粉中綠原痠的含量。方法：採用Agilent Eclipse Plus C18（5μm ,4.6×250mm）色譜柱，流動相為乙腈-0.1%燐痠水溶液（10∶90，V/V），檢測波長為348nm，流速1.0mL/min，進樣量20μL。結果：綠原痠54.375～870mg/L範圍呈線性，相關繫數R為0.9998，該方法重複性及迴收率均符閤要求。結論：本法用于測定菊花超細粉中綠原痠的含量測定，具有簡便、準確、高效等特點，可為鑒彆菊花超微粉的真偽提供一定科學依據。
목적：채용HPLC법측정국화초세분중록원산적함량。방법：채용Agilent Eclipse Plus C18（5μm ,4.6×250mm）색보주，류동상위을정-0.1%린산수용액（10∶90，V/V），검측파장위348nm，류속1.0mL/min，진양량20μL。결과：록원산54.375～870mg/L범위정선성，상관계수R위0.9998，해방법중복성급회수솔균부합요구。결론：본법용우측정국화초세분중록원산적함량측정，구유간편、준학、고효등특점，가위감별국화초미분적진위제공일정과학의거。
Objective: This study has been to establish a method for determination of Chlorogenic acid in Chrysanthemum Ultrafine powder. Method: Agilent Eclipse Plus C18 (5μm, 4.6×250mm) chromatographic column was used, with mixtures of acetonitrile and Phosphoric acid solution as mobile phase (10∶90, V/V), the wavelength was 348nm, the flow rate was 1. 0mL/min, the injection volume was 20μL. Results: The linear range of Chlorogenic acid is 54.375~870mg/L, and the correlation coefficient R is 0.9998, repeatability and recovery rate are good. Conclusion:The presented method is a simple, accurate, efficient method for determination of Chlorogenic acid in Chrysanthemum Ultrafine powder.
