CAJ | 학술논문

目的：探寻乌拉坦诱导肺癌形成过程中 lncRNA 表达的动态变化过程。方法BALB/c 小鼠每周 ip 给予乌拉坦1 g·kg -1，连续4周，分别在处理后第12周或第24周处死。分离小鼠肺组织，提取 RNA。通过长链非编码 RNA 芯片和定量 PCR 的方法分析长链非编码 RNA-AK017233表达。结果与对照组相比，乌拉坦染毒12周后的小鼠肺肿瘤的发生率为85％，每只小鼠平均肿瘤数为2．81±2．04，肿瘤的平均大小为（0．68±0．20）mm；乌拉坦染毒24周后的小鼠肺肿瘤的发生率为100％，每只小鼠平均肿瘤数为6．00±2．07，肿瘤的平均大小为（1．76±1．34）mm。病理检查显示均为肺腺癌。乌拉坦处理的确可以影响小鼠肺组织内的长链非编码 RNA 表达情况。通过芯片分析，发现以长链非编码 RNA-AK017233为代表的26个表达下调的长链非编码 RNA，随后通过定量 PCR 确认长链非编码 RNA-AK017233在乌拉坦处理12周诱发的肺腺癌组织中表达水平下调33％，在乌拉坦处理24周诱发的肺腺癌组织中表达水平下调22％。结论长链非编码 RNA-AK017233在乌拉坦诱导的肺癌形成过程中始终处于低表达状态。
목적：탐심오랍탄유도폐암형성과정중 lncRNA 표체적동태변화과정。방법BALB/c 소서매주 ip 급여오랍탄1 g·kg -1，련속4주，분별재처리후제12주혹제24주처사。분리소서폐조직，제취 RNA。통과장련비편마 RNA 심편화정량 PCR 적방법분석장련비편마 RNA-AK017233표체。결과여대조조상비，오랍탄염독12주후적소서폐종류적발생솔위85％，매지소서평균종류수위2．81±2．04，종류적평균대소위（0．68±0．20）mm；오랍탄염독24주후적소서폐종류적발생솔위100％，매지소서평균종류수위6．00±2．07，종류적평균대소위（1．76±1．34）mm。병리검사현시균위폐선암。오랍탄처리적학가이영향소서폐조직내적장련비편마 RNA 표체정황。통과심편분석，발현이장련비편마 RNA-AK017233위대표적26개표체하조적장련비편마 RNA，수후통과정량 PCR 학인장련비편마 RNA-AK017233재오랍탄처리12주유발적폐선암조직중표체수평하조33％，재오랍탄처리24주유발적폐선암조직중표체수평하조22％。결론장련비편마 RNA-AK017233재오랍탄유도적폐암형성과정중시종처우저표체상태。
OBJECTIVE To explore the dyna mic change of lncRNA expression during lung carcino-genesis induced by urethane.METHODS A total of 40 BALB/c mice received weekly ip injection of urethane 1 g·kg -1 for four continuous weeks,mice were euthanized at 12th week or 24th week after the first urethane treat ment,respectively.The RNA of lung tissues were isolated and used for microarray analysis.Based on the results of the microarray we selected lncRNA-AK017233 for additional qPCR analysis in individual mouse.RESULTS The incidence of lung cancer were 85% and 100% at 12th week and 24th week after the first ad ministration of urethane,respectively.The multiplicity and dia meter of lung tu mors in 24 weeks treated group were statistically significant fro m those in 12 weeks treated group (P<0.01 ),and pathological analysis showed that tu mors were classifiable as moderately differ-entiated adenocarcino ma.Total of 26 Down-regulated lncRNAs in which lncRNA-AK017233 stand for the most down-regulated lncRNA were identified by microcarray analysis.qPCR detected that the lncRNA-AK017233 was significantly altered by 0.33 ti mes in lung tu mors of urethane treated group at 12th week, co mpared to parallel lung tissues in urethane treated group at 12th week.AK017233 expression of ure-thane treated group was significantly reduced by 0.22 ti mes at 24th week,co mpared to parallel lung tis-sues in urethane treated group at 24th week.CONCLUSION LncRNA-AK017233 was consistently down-regulated during urethane induced lung carcinogenesis.