云南中医学院学报
雲南中醫學院學報
운남중의학원학보
JOURNAL OF YUNNAN COLLEGE OF TRADITIONAL CHINESE MEDICINE
2014年
2期
14-16
,共3页
张文平%张贵华%柯瑾%马云淑
張文平%張貴華%柯瑾%馬雲淑
장문평%장귀화%가근%마운숙
马钱子%总生物碱%正交试验%提取工艺
馬錢子%總生物堿%正交試驗%提取工藝
마전자%총생물감%정교시험%제취공예
Semen Strychni%total alkaloids of Semen Strychni%orthogonal test%extraction technology
目的:优选马钱子中士的宁与马钱子碱的提取方法与工艺。方法在单因素试验的基础上,以高效液相测定的士的宁与马钱子碱总量为指标,以乙醇浓度、乙醇用量及回流时间为影响因素,采用正交试验法优选士的宁与马钱子碱提取工艺。结果优选的最佳提取工艺为50%乙醇10倍量,回流提取3次,每次2h。结论验证实验表明该工艺合理、稳定可行。
目的:優選馬錢子中士的寧與馬錢子堿的提取方法與工藝。方法在單因素試驗的基礎上,以高效液相測定的士的寧與馬錢子堿總量為指標,以乙醇濃度、乙醇用量及迴流時間為影響因素,採用正交試驗法優選士的寧與馬錢子堿提取工藝。結果優選的最佳提取工藝為50%乙醇10倍量,迴流提取3次,每次2h。結論驗證實驗錶明該工藝閤理、穩定可行。
목적:우선마전자중사적저여마전자감적제취방법여공예。방법재단인소시험적기출상,이고효액상측정적사적저여마전자감총량위지표,이을순농도、을순용량급회류시간위영향인소,채용정교시험법우선사적저여마전자감제취공예。결과우선적최가제취공예위50%을순10배량,회류제취3차,매차2h。결론험증실험표명해공예합리、은정가행。
Objective To optimize extraction process conditions of Semen Strychni. Methods With the content of Strychnine and brucine as the target,based on the single factor experiment,extraction technology of Semen Strychni was optimized by L9(34) orthogonal test,taking the concentration of ethanol,ethanol dosage and extraction time as investigated factors. Results Optimum extraction technology was:extracted 3 times with 10 times the amount of 50%ethanol,2 hours each time. Conclusion This optimized extraction technology was simple and reasonable and it was stable.