中国药物应用与监测
中國藥物應用與鑑測
중국약물응용여감측
CHINESE JOURNAL OF DRUG APPLICATION AND MONITORING
2014年
2期
82-84
,共3页
蔡乐%白林%徐风华%任韡%王欢欢
蔡樂%白林%徐風華%任韡%王歡歡
채악%백림%서풍화%임위%왕환환
薄荷苯酚软膏%苯酚%高效液相色谱法%含量测定
薄荷苯酚軟膏%苯酚%高效液相色譜法%含量測定
박하분분연고%분분%고효액상색보법%함량측정
Menthol-phenol ointment%Phenol%HPLC%Content determination
目的：建立HPLC法测定薄荷苯酚软膏中苯酚的含量。方法：采用超声提取方法处理薄荷苯酚软膏，用HPLC法进行含量测定。色谱柱：Diamonsil C18柱（250 mm ×4.6 mm，5μm），流动相：甲醇-水（60∶40），流速：1.0 mL·min-1，检测波长：271 nm，柱温：45℃。结果：苯酚在9~360μg·mL-1范围内线性关系良好（r=0.9999）；样品平均回收率为100.2%，RSD为0.68%（n=6）。结论：本方法简便、准确、重复性好，可用于薄荷苯酚软膏中苯酚的含量测定。
目的：建立HPLC法測定薄荷苯酚軟膏中苯酚的含量。方法：採用超聲提取方法處理薄荷苯酚軟膏，用HPLC法進行含量測定。色譜柱：Diamonsil C18柱（250 mm ×4.6 mm，5μm），流動相：甲醇-水（60∶40），流速：1.0 mL·min-1，檢測波長：271 nm，柱溫：45℃。結果：苯酚在9~360μg·mL-1範圍內線性關繫良好（r=0.9999）；樣品平均迴收率為100.2%，RSD為0.68%（n=6）。結論：本方法簡便、準確、重複性好，可用于薄荷苯酚軟膏中苯酚的含量測定。
목적：건립HPLC법측정박하분분연고중분분적함량。방법：채용초성제취방법처리박하분분연고，용HPLC법진행함량측정。색보주：Diamonsil C18주（250 mm ×4.6 mm，5μm），류동상：갑순-수（60∶40），류속：1.0 mL·min-1，검측파장：271 nm，주온：45℃。결과：분분재9~360μg·mL-1범위내선성관계량호（r=0.9999）；양품평균회수솔위100.2%，RSD위0.68%（n=6）。결론：본방법간편、준학、중복성호，가용우박하분분연고중분분적함량측정。
Objective:To establish the HPLC method for determination of phenol in menthol-phenol ointment. Methods:The phenol in menthol-phenol ointment was extracted by ultrasonic method, and the content of phenol was determined by HPLC method. The analysis was carried out on a column of Diamonsil C18 (250 mm × 4.6 mm, 5μm) with mobile phase consisting of methanol-water (60∶40), the lfow rate was 1.0 mL·min-1. The detection wavelength was set at 271 nm, and the column temperature was 45℃. Results:Phenol showed a good linear relationship in the range of 9-360μg·mL-1 (r=0.999 9). The average recovery was 100.2%with RSD of 0.68%(n=6). Conclusion: The method is simple, accurate and repeatable, which is suitable for the content determination of phenol in menthol-phenol ointment.
