CAJ | 학술논문

目的：探索知母皂苷B-Ⅱ纳米纤维膜的制备方法，为后续进行体内相关研究打好基础。方法：将知母皂苷B-Ⅱ与聚乳酸（PLLA）按照不同比例制成知母皂苷B-Ⅱ纳米纤维膜；分别采用扫描电镜、红外光谱分析、LC-MS/MS检测方法对纤维膜理化性质表征、生物相容性、缓释性能等进行测试；采用CCK-8法检测载药纳米膜缓释药物抑制胃癌细胞的增殖情况。结果：12%的知母皂苷B-Ⅱ浓度为最佳载药浓度；在制备载知母皂苷B-Ⅱ纳米纤维的过程中，静电纺丝技术能保持两种组分各自的化学特征及物理性质；知母皂苷B-Ⅱ对纤维膜的热稳定性影响不大，两者相容性较好；知母皂苷B-Ⅱ纳米纤维膜可有效释放知母皂苷B-Ⅱ，明显抑制BGC-823细胞的增殖活性，在48 h、72 h与对照组细胞相比较，差异具有统计学意义（P<0.05）。结论：知母皂苷B-Ⅱ纳米纤维膜通过缓释药物可有效抑制BGC-823细胞的增殖能力。
목적：탐색지모조감B-Ⅱ납미섬유막적제비방법，위후속진행체내상관연구타호기출。방법：장지모조감B-Ⅱ여취유산（PLLA）안조불동비례제성지모조감B-Ⅱ납미섬유막；분별채용소묘전경、홍외광보분석、LC-MS/MS검측방법대섬유막이화성질표정、생물상용성、완석성능등진행측시；채용CCK-8법검측재약납미막완석약물억제위암세포적증식정황。결과：12%적지모조감B-Ⅱ농도위최가재약농도；재제비재지모조감B-Ⅱ납미섬유적과정중，정전방사기술능보지량충조분각자적화학특정급물이성질；지모조감B-Ⅱ대섬유막적열은정성영향불대，량자상용성교호；지모조감B-Ⅱ납미섬유막가유효석방지모조감B-Ⅱ，명현억제BGC-823세포적증식활성，재48 h、72 h여대조조세포상비교，차이구유통계학의의（P<0.05）。결론：지모조감B-Ⅱ납미섬유막통과완석약물가유효억제BGC-823세포적증식능력。
Objective: To explore the preparation method of timosaponin B-Ⅱnanofiber membrane, in order to lay a foundation for the subsequent studies in vivo. Methods:Timosaponin B-Ⅱand PLLA at different ratios were made into timosaponin nanoifber membranes;the surface structure and morphology of the drug loaded nanoifber membranes were observed by scanning electron microscopy;the infrared spectra was adopted to determine the compatibility between the drug and the polymer;LC-MS/MS was used to measure timosaponin B-Ⅱlevels in solutions at different time points to draw the drug release curves;CCK-8 assay was used to detect the proliferative activity of tumor cells, in order to evaluate whether the sustained drug release from the drug-loaded nano-iflms could inhibit the proliferation of gastric carcinoma cells. Results: The optimum concentration of timosaponin B-Ⅱfor drug loading was 12%; the electrospinning technique in the preparation of timosaponin B-Ⅱloaded nanofibers could maintain the physical and chemical properties of the both components; timosaponin B-Ⅱhad little effect on the thermal stability of fiber membranes, indicating a good compatibility;the timosaponin B-Ⅱnanoifber membranes could effectively release timosaponin B-Ⅱin the conventional culture solution, the timosaponin B-Ⅱloaded nanoifber membranes can inhibit the proliferation of BGC-823 cells signiifcantly, the differences in the treated groups at 48 hours and 72 hours were signiifcant (P<0.05) compared with the control group. Conclusion:Timosaponin B-Ⅱnanoifber membranes can effectively inhibit the proliferation of BGC-823 cells through the sustained release of the drug.