CAJ | 학술논문

　　用单因素分析法研究在培养基中缺乏铁、镁、微量元素液、维生素液时对阴沟肠杆菌 XA-2生长的影响。实验发现,铁和镁对该菌的生长影响显著,而缺乏微量元素液、维生素液对该菌的生长影响不明显。缺铁培养时该菌在稳定期的菌浊浓度 OD600nm为1.2586,缺镁培养时菌浊浓度为0.6988,仅为完全培养时菌浊浓度的三分之一,其中,镁对该菌生长的影响更显著。产氢实验表明,各因素对产氢情况的影响显著,依次为：铁>镁>维生素液>微量元素液。当该菌利用缺铁培养基产氢时,其累计产氢量仅为44 mL/L ,铁元素的缺乏严重影响该菌的产氢活动。
　　용단인소분석법연구재배양기중결핍철、미、미량원소액、유생소액시대음구장간균 XA-2생장적영향。실험발현,철화미대해균적생장영향현저,이결핍미량원소액、유생소액대해균적생장영향불명현。결철배양시해균재은정기적균탁농도 OD600nm위1.2586,결미배양시균탁농도위0.6988,부위완전배양시균탁농도적삼분지일,기중,미대해균생장적영향경현저。산경실험표명,각인소대산경정황적영향현저,의차위：철>미>유생소액>미량원소액。당해균이용결철배양기산경시,기루계산경량부위44 mL/L ,철원소적결핍엄중영향해균적산경활동。
The consequences of lack of iron, magnesium, trace nutrients and vitamin nutrients solution on the cell growth of Enterobacter cloacae XA-2 was researched by using single factor test. The results showed that iron and magnesium had significant effect on the growth of bacteria. Deficiency of trace nutrients, vitamin nutrients solution in medium had little effect on the growth of the strain. The absorbance at 600 nm of the strain in stationary phase was 1.2586 without iron and 0.6988 without magnesium in medium. The magnesium absence value was only one-third of complete medium. Compared with iron, magnesium had more significant effect on the growth of the bacterium. Hydrogen production experiments showed that the influence order of factors on hydrogen production was as follow:Fe>Mg>Vitamin nutrients>trace nutrients. In iron absence medium, cumulative hydrogen production was 44 mL/L medium. Iron deficiency had significant effect on hydrogen production of the strain.